Multiphoton single-molecule localization by sequential excitation with light minima

Luciano A. Masullo; Fernando D. Stefani

doi:10.1038/s41377-022-00763-2

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Multiphoton single-molecule localization by sequential excitation with light minima

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- Multiphoton single-molecule localization by sequential excitation with light minima
- Light: Science & Applications Vol. 11, Issue 4, Pages: 462-465(2022)
- Affiliations：
  
  1.Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
  2.Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Güiraldes 2620, C1428EHA Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
  3.Present address: Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, .Germany
- Author bio：
  
  Fernando D. Stefani (fernando.stefani@df.uba.ar)
- Funds：
- DOI：10.1038/s41377-022-00763-2
  CLC：
- Published：30 April 2022，
  
  Published Online：25 March 2022，
- Accepted：
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Masullo, L. A. & Stefani, F. D. Multiphoton single-molecule localization by sequential excitation with light minima. Light: Science & Applications, 11, 462-465 (2022).
DOI：

Masullo, L. A. & Stefani, F. D. Multiphoton single-molecule localization by sequential excitation with light minima. Light: Science & Applications, 11, 462-465 (2022). DOI： 10.1038/s41377-022-00763-2.

摘要

Abstract

Using sequential excitation with a minimum of light to localize single fluorescent molecules represented a breakthrough because it delivers 1–2 nm precision with moderate photon counts

enabling tracking and super-resolution imaging with true molecular resolution. Expanding this concept to multi-photon regimes may be a useful complement to reach even higher localization precision and get deeper into biological specimens.

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references

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