Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry

Maciej Trusiak

doi:10.1038/s41377-025-02053-z

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Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry

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- Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry
- Light: Science & Applications Vol. 14, Issue 12, Pages: 3793-3794(2025)
- Affiliations：
  
  Warsaw University of Technology, Institute of Micromechanics and Photonics, Faculty of Mechatronics, Warsaw, Poland
- Author bio：
  
  Maciej Trusiak (maciej.trusiak@pw.edu.pl)
- Funds：
- DOI：10.1038/s41377-025-02053-z
  CLC：
- Published Online：14 October 2025，
  
  Published：31 December 2025
- Accepted：
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Trusiak, M. Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry. Light: Science & Applications, 14, 3793-3794 (2025).
DOI：

Trusiak, M. Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry. Light: Science & Applications, 14, 3793-3794 (2025). DOI： 10.1038/s41377-025-02053-z.

摘要

Abstract

Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated. A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies

with virtual reality visualization offering engaging immersion. The method enables population-level detection of statistically significant shifts in 3D cell morphology

originally correlating phenotype with genotype.

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references

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