Near infrared light controlled gene editing

Mikhail Y. Berezin

doi:10.1038/s41377-025-02128-x

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Near infrared light controlled gene editing

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- Near infrared light controlled gene editing
- Light: Science & Applications Vol. 15, Issue 3, Pages: 707-709(2026)
- Affiliations：
  
  Washington University School of Medicine, St. Louis, MO 63110, USA
- Author bio：
  
  Mikhail Y. Berezin (berezinm@wustl.edu)
- Funds：
- DOI：10.1038/s41377-025-02128-x
  CLC：
- Online First：05 January 2026，
  
  Published：31 March 2026
- Accepted：
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Berezin, M. Y. Near infrared light controlled gene editing. Light: Science & Applications, 15, 707-709 (2026).
DOI：

Berezin, M. Y. Near infrared light controlled gene editing. Light: Science & Applications, 15, 707-709 (2026). DOI： 10.1038/s41377-025-02128-x.

摘要

Abstract

A novel NIR light-activated CRISPR-dCas9/Cas9 system achieves precise and rapid gene regulation in living organism using a chemically cleavable rapamycin dimer. Unlike previous light-driven systems

this approach offers deeper tissue penetration

low toxicity

fast response

and minimal background activity. This platform opens new directions for highly efficient

targeted

noninvasive

and spatially confined gene editing for a great number of preclinical and clinically translatable applications.

关键词

Keywords

references

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Zhang, L. et al. Near-infrared light activatable chemically induced CRISPR system. Light Sci. Appl. 14 , 229 (2025)..

Nihongaki, Y. et al. Photoactivatable CRISPR-Cas9 for optogenetic genome editing. Nat. Biotechnol. 33 , 755–760 (2015)..

Hu, M. L. et al. Scalable modulation of CRISPR‒Cas enzyme activity using photocleavable phosphorothioate DNA. Nat. Commun. 16 , 5939 (2025)..

Liu, Y. et al. Very fast CRISPR on demand. Science 368 , 1265–1269 (2020)..

Zhou, W. Y. et al. Spatiotemporal control of CRISPR/Cas9 function in cells and zebrafish using light-activated guide RNA. Angew. Chem. Int. Ed. 59 , 8998–9003 (2020)..

Hemphill, J. et al. Optical control of CRISPR/Cas9 gene editing. J. Am. Chem. Soc. 137 , 5642–5645 (2015)..

Berezin, M. Y. et al. Long fluorescence lifetime molecular probes based on near infrared pyrrolopyrrole cyanine fluorophores for in vivo imaging. Biophys. J. 97 , L22–L24 (2009)..

Frangioni, J. V. In vivo near-infrared fluorescence imaging. Curr. Opin. Chem. Biol. 7 , 626–634 (2003)..

Yu, Y. H. et al. Engineering a far-red light-activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors. Sci. Adv. 6 , eabb1777 (2020)..

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