Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level

Zhen Ma; Qiuying Liu; Huaxiao Yang; Raymond B Runyan; Carol A Eisenberg; Meifeng Xu; Thomas K Borg; Roger Markwald; Yifei Wang; Bruce Z Gao

doi:10.1038/lsa.2013.24

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Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level

Original Articles

- Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level
- Light: Science & Applications Vol. 2, Issue 1-2, (2013)
- Affiliations：
  
  1.Department of Bioengineering and COMSET, Clemson University, Clemson, SC 29634, USA
  2.Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA
  3.Biomedical R&D Center, Jinan University, Guangzhou, China
  4.Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA
  5.New York Medical College/Westchester Medical Center Stem Cell Laboratory, New York Medical College, Valhalla, New York, USA
  6.Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, OH, USA
  7.Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA
- Author bio：
  
  Professor BZ Gao, Department of Bioengineering and COMSET, Clemson University, 201-3 Rhodes Hall, Clemson, SC 29634, USA Email: zgao@clemson.edu
- Funds：
- DOI：10.1038/lsa.2013.24
  CLC：
- Published：2013-06，
  
  Published Online：24 May 2013，
  
  Received：22 September 2012，
  
  Revised：03 January 2013，
  
  Accepted：07 January 2013
- Accepted：
Scan QR Code
Ma, Z. et al. Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level. Light: Science & Applications, 2, e68 (2013).
DOI：

Ma, Z. et al. Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level. Light: Science & Applications, 2, e68 (2013). DOI： 10.1038/lsa.2013.24.

摘要

Abstract

Mesenchymal stem cells (MSCs) have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions

including the paracrine effect

cell fusion

and mechanical and electrical couplings. Due to heart–muscle complexity

progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture. In conventional coculture systems

however

the

in vivo

cardiac muscle structure

in which rod-shaped cells are connected end-to-end

is not sustained; instead

irregularly shaped cells spread randomly

resulting in randomly distributed cell junctions. Consequently

contact-mediated cell–cell interactions (e.g.

the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions) occur randomly. Thus

the data generated on the beneficial effects of MSCs may be irrelevant to

in vivo

biological processes. In this study

we explored whether cardiomyocyte alignment

the most important phenotype

is relevant to stem cell cardiogenic differentiation. Here

we report (ⅰ) the construction of a laser-patterned

biochip-based

stem cell–cardiomyocyte coculture model with controlled cell alignment; and (ⅱ) single-cell-level data on stem cell cardiogenic differentiation under

in vivo

-like cardiomyocyte alignment conditions.

关键词

Keywords

cardiogenic differentiationmesenchymal stem cellsmicroenvironmentoptical forcesingle-cell analysis

references

Ruiz SA, Chen CS. Emergence of patterned stem cell differentiation within multicellular structures.Stem Cells2008;26: 2921–2927..

Rowlands AS, George PA, Cooper-White JJ. Directing osteogenic and myogenic differentiation of MSCs: interplay of stiffness and adhesive ligand presentation.Am J Physiol Cell Physiol2008;295: C1037–C1044..

Kilian KA, Bugarija B, Lahn BT, Mrksich M. Geometric cues for directing the differentiation of mesenchymal stem cells.Proc Natl Acad Sci USA2010;107: 4872–4877..

Sung JH, Shuler ML. Microtechnology for mimickingin vivotissue environment.Ann Biomed Eng2012;40: 1289–1300..

Park JY, Takayama S, Lee SH. Regulating microenvironmental stimuli for stem cells and cancer cells using microsystems.Integr Biol2010;2: 229–240..

Scanlon MJ, Ohtsu K, Timmermans MC, Schnable PS. Laser microdissection-mediated isolation andin vitrotranscriptional amplification of plant RNA.Curr Protoc Mol Biol2009;Chapter 25: Unit 25A 3..

Li Y, Thompson H, Hemphill C, Hong F, Forrester Jet al. An improved one-tube RT-PCR protocol for analyzing single-cell gene expression in individual mammalian cells.Anal Bioanal Chem2010;397: 1853–1859..

Di Carlo D, Wu LY, Lee LP. Dynamic single cell culture array.Lab Chip2006;6: 1445–1449..

Sussman MA, Murry CE. Bones of contention: marrow-derived cells in myocardial regeneration.J Mol Cell Cardiol2008;44: 950–953..

Laflamme MA, Zbinden S, Epstein SE, Murry CE. Cell-based therapy for myocardial ischemia and infarction: pathophysiological mechanisms.Annu Rev Pathol2007;2: 307–339..

Pittenger MF, Martin BJ. Mesenchymal stem cells and their potential as cardiac therapeutics.Circ Res2004;95: 9–20..

Pijnappels DA, Schalij MJ, Ramkisoensing AA, van Tuyn J, de Vries AAet al. Forced alignment of mesenchymal stem cells undergoing cardiomyogenic differentiation affects functional integration with cardiomyocyte cultures.Circ Res2008;103: 167–176..

Klinger R, Bursac N. Cardiac cell therapyin vitro: reproducible assays for comparing the efficacy of different donor cells.IEEE Eng Med Biol Mag2008;27: 72–80..

Pedrotty DM, Klinger RY, Badie N, Hinds S, Kardashian Aet al. Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay.Am J Physiol Heart Circ Physiol2008;295: H390–H400..

Pirlo RK, Ma Z, Sweeney A, Liu H, Yun JXet al. Laser-guided cell micropatterning system.Rev Sci Instrum2011;82: 013708..

Geisse NA, Sheehy SP, Parker KK. Control of myocyte remodelingin vitrowith engineered substrates.In Vitro Cell Dev Biol Anim2009;45: 343–350..

Ma Z, Pirlo RK, Wan Q, Yun JX, Yuan Xet al. Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning.Biofabrication2011;3: 034107..

Ashkin A. Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime.Methods Cell Biol1998;55: 1–27..

Ren KF, Grehan G, Gouesbet G. Prediction of reverse radiation pressure by generalized Lorenz–Mie theory.Appl Opt1996;35: 2702–2710..

Nahmias YK, Gao BZ, Odde DJ. Dimensionless parameters for the design of optical traps and laser guidance systems.Appl Opt2004;43: 3999–4006..

Ma Z, Liu Q, Liu H, Yang H, Yun JXet al. Laser-patterned stem-cell bridges in a cardiac muscle model for on-chip electrical conductivity analyses.Lab Chip2012;12: 566–573..

Nahmias Y, Odde DJ. Analysis of radiation forces in laser trapping and laser-guided direct writing applications.IEEE J Quantum Electron2002;38: 131–141..

de Boer TP, van Veen TA, Jonsson MK, Kok BG, Metz CHet al. Human cardiomyocyte progenitor cell-derived cardiomyocytes display a maturated electrical phenotype.J Mol Cell Cardiol2010;48: 254–260..

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