Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy

Doekele G Stavenga; Hein L Leertouwer; Bodo D Wilts

doi:10.1038/lsa.2013.56

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Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy

Original Articles

- Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy
- Light: Science & Applications Vol. 2, Issue 3-4, (2013)
- Affiliations：
  
  Computational Physics, Zernike Institute for Advanced Materials, University of Groningen, NL-9747 AG Groningen, The Netherlands
- Author bio：
  
  Professor DG Stavenga, Computational Physics, Zernike Institute for Advanced Materials, University of Groningen, NL-9747 AG Groningen, The Netherlands E-mail: D.G.Stavenga@rug.nl
- Funds：
- DOI：10.1038/lsa.2013.56
  CLC：
- Published：2013-12，
  
  Published Online：27 September 2013，
  
  Received：25 March 2013，
  
  Revised：28 May 2013，
  
  Accepted：30 May 2013
- Accepted：
Scan QR Code
Stavenga, D. G., Leertouwer, H. L. & Wilts, B. D. Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy. Light: Science & Applications, 2, e100 (2013).
DOI：

Stavenga, D. G., Leertouwer, H. L. & Wilts, B. D. Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy. Light: Science & Applications, 2, e100 (2013). DOI： 10.1038/lsa.2013.56.

摘要

Abstract

Jamin–Lebedeff polarizing interference microscopy is a classical method for determining the refractive index and thickness of transparent tissues. Here

we extend the application of this method to pigmented

absorbing biological tissues

based on a theoretical derivation using Jones calculus. This novel method is applied to the wings of the American Rubyspot damselfly

Hetaerina americana

. The membranes in the red-colored parts of the damselfly's wings

with a thickness of ~2.5 µm

contain a pigment with maximal absorption at ~490 nm and a peak absorbance coefficient of ~0.7 µm

−1

. The high pigment density causes a considerable and anomalous dispersion of the refractive index. This result can be quantitatively understood from the pigment absorbance spectrum by applying the Kramers–Kronig dispersion relations. Measurements of the spectral dependence of the refractive index and the absorption are valuable for gaining quantitative insight into how the material properties of animal tissues influence coloration.

关键词

Keywords

absorptionchitininsect wingsJones calculusKramers–Kronig relations

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