Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy

Lin Li; Wei Guo; Yinzhou Yan; Seoungjun Lee; Tao Wang

doi:10.1038/lsa.2013.60

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Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy

Original Articles

- Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy
- Light: Science & Applications Vol. 2, Issue 3-4, (2013)
- Affiliations：
  
  1.Laser Processing Research Centre, School of Mechanical, Aerospace and Civil Engineering, and Photon Science Institute, The University of Manchester, Manchester M13 9PL, UK
  2.Faculty of Medical and Human Sciences, The University of Manchester, Manchester M13 9PL, UK
- Author bio：
  
  Professor L Li, Laser Processing Research Centre, School of Mechanical, Aerospace and Civil Engineering, and Photon Science Institute, The University of Manchester, Manchester M13 9PL, UK E-mail: lin.li@manchester.ac.uk
- Funds：
- DOI：10.1038/lsa.2013.60
  CLC：
- Published：2013-12，
  
  Published Online：27 September 2013，
  
  Received：29 August 2012，
  
  Revised：24 June 2013，
  
  Accepted：25 June 2013
- Accepted：
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Li, L. et al. Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy. Light: Science & Applications, 2, e104 (2013).
DOI：

Li, L. et al. Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy. Light: Science & Applications, 2, e104 (2013). DOI： 10.1038/lsa.2013.60.

摘要

Abstract

Because of the small sizes of most viruses (typically 5–150 nm)

standard optical microscopes

which have an optical diffraction limit of 200 nm

are not generally suitable for their direct observation. Electron microscopes usually require specimens to be placed under vacuum conditions

thus making them unsuitable for imaging live biological specimens in liquid environments. Indirect optical imaging of viruses has been made possible by the use of fluorescence optical microscopy that relies on the stimulated emission of light from the fluorescing specimens when they are excited with light of a specific wavelength

a process known as labeling or self-fluorescent emissions from certain organic materials. In this paper

we describe direct white-light optical imaging of 75-nm adenoviruses by submerged microsphere optical nanoscopy (SMON) without the use of fluorescent labeling or staining. The mechanism involved in the imaging is presented. Theoretical calculations of the imaging planes and the magnification factors have been verified by experimental results

with good agreement between theory and experiment.

关键词

Keywords

imagingmicroscopeopticalsuper-resolutionvirus

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