Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter

Andreu Llobera; Joan Juvert; Alfredo González-Fernández; Bergoi Ibarlucea; Ester Carregal-Romero; Stephanus Büttgenbach; César Fernández-Sánchez

doi:10.1038/lsa.2015.44

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Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter

Original Articles

- Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter
- Light: Science & Applications Vol. 4, Issue 2, (2015)
- Affiliations：
  
  1.Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC), Campus UAB, 08193 Bellaterra, Spain
  2.Institut für Mikrotechnik, Technische Universität Braunschweig, Braunschweig 38106, Germany
- Author bio：
  
  A Llobera, Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC), Campus UAB, 08193 Bellaterra, Spain Email: andreu.llobera@imb-cnm.csic.es
  C Fernández-Sánchez, Instituto de Microelectro´ nica de Barcelona IMB-CNM (CSIC), Campus UAB, 08193 Bellaterra, Spain Email: cesar.fernandez@imb-cnm.csic.es
- Funds：
- DOI：10.1038/lsa.2015.44
  CLC：
- Published：2015-06，
  
  Published Online：20 January 2015，
  
  Received：23 October 2014，
  
  Revised：16 December 2014，
  
  Accepted：10 January 2015
- Accepted：
Scan QR Code
Llobera, A. et al. Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter. Light: Science & Applications, 4, e271 (2015).
DOI：

Llobera, A. et al. Biofunctionalized all-polymer photonic lab on a chip with integrated solid-state light emitter. Light: Science & Applications, 4, e271 (2015). DOI： 10.1038/lsa.2015.44.

摘要

Abstract

A photonic lab on a chip (PhLOC)

comprising a solid-state light emitter (SSLE) aligned with a biofunctionalized optofluidic multiple internal reflection (MIR) system

is presented. The SSLE is obtained by filling a microfluidic structure with a phenyltrimethoxysilane (PhTMOS) aqueous

sol

solution containing a fluorophore organic dye. After curing

the resulting xerogel solid structure retains the emitting properties of the fluorophore

which is evenly distributed in the xerogel matrix. Photostability studies demonstrate that after a total dose (at

=365 nm) greater than 24 J cm

−2

the xerogel emission decay is only 4.1%. To re-direct the emitted light

the SSLE includes two sets of air mirrors that surround the xerogel. Emission mapping of the SSLE demonstrates that alignment variations of 150 µm (between the SSLE and the external pumping light source) provide fluctuations in emitted light smaller than 5%. After this verification

the SSLE is monolithically implemented with a MIR

forming the PhLOC. Its performance is assessed by measuring quinol

one yellow

obtaining a limit of detection (LOD) of (0.60±0.01) µM. Finally

the MIR is selectively biofunctionalized with horseradish peroxidase (HRP) for the detection of hydrogen peroxide (H

) target analyte

obtaining a LOD of (0.7±0.1) µM for H

confirming

for the first time

that solid-state xerogel-based emitters can be massively implemented in biofunctionalized PhLOCs.

关键词

Keywords

medical devicesphotonic lab on a chippolymer technologysolid-state light emitterssol–gel

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