Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy

Tobias Steinle; Vikas Kumar; Moritz Floess; Andy Steinmann; Marco Marangoni; Claudia Koch; Christina Wege; Giulio Cerullo; Harald Giessen

doi:10.1038/lsa.2016.149

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Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy

Original Articles

- Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy
- Light: Science & Applications Vol. 5, Issue 4, (2016)
- Affiliations：
  
  1.4th Physics Institute and Research Center SCoPE, University of Stuttgart, D-70569, Stuttgart, Germany
  2.IFN-CNR, Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, I-20133, Milan, Italy
  3.Institute of Biomaterials and Biomolecular Systems, Dpt. of Molecular Biology and Plant Virology, University of Stuttgart, Pfaffenwaldring 57, D-70569, Stuttgart, Germany
- Author bio：
  
  T Steinle, Email: t.steinle@pi4.uni-stuttgart.de
- Funds：
- DOI：10.1038/lsa.2016.149
  CLC：
- Published：2016-12，
  
  Published Online：07 April 2016，
  
  Received：22 December 2015，
  
  Revised：01 April 2016，
  
  Accepted：05 April 2016
- Accepted：
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Steinle, T. et al. Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy. Light: Science & Applications, 5, e16149 (2016).
DOI：

Steinle, T. et al. Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy. Light: Science & Applications, 5, e16149 (2016). DOI： 10.1038/lsa.2016.149.

摘要

Abstract

We introduce an extremely simple and highly stable system for stimulated Raman scattering (SRS) microscopy. An 8-W

450-fs Yb: KGW bulk oscillator with 41 MHz repetition rate pumps an optical parametric amplifier

which is seeded by a cw tunable external cavity diode laser. The output radiation is frequency doubled in a long PPLN crystal and generates 1.5-ps long narrowband pump pulses that are tunable between 760 and 820 nm with > 50 mW average power. Part of the oscillator output is sent through an etalon and creates Stokes pulses with 100 mW average power and 1.7 ps duration. We demonstrate SRS microscopy at a 30-μs pixel dwell time with high chemical contrast

signal-to-noise ratio in excess of 45 and no need for balanced detection

thanks to the favorable noise properties of the bulk solid-state system. Cw seeding intrinsically ensures low spectral drift. We discuss its application to chemical contrast microscopy of freshly prepared plant tissue sections at different vibrational bands.

关键词

Keywords

label-free imagingstimulated Raman scatteringoptical parametric amplifierspectral compression

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