Optical disassembly of cellular clusters by tunable 'tug-of-war' tweezers

Anna S Bezryadina; Daryl C Preece; Joseph C Chen; Zhigang Chen

doi:10.1038/lsa.2016.158

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Optical disassembly of cellular clusters by tunable 'tug-of-war' tweezers

Original Articles

- Optical disassembly of cellular clusters by tunable 'tug-of-war' tweezers
- Light: Science & Applications Vol. 5, Issue 4, (2016)
- Affiliations：
  
  1.Department of Physics and Astronomy, San Francisco State University, San Francisco, CA 94132, USA
  2.School of Mathematics and Physics, The University of Queensland, Brisbane, QLD 4072, Australia
  3.Department of NanoEngineering, University of California, San Diego, La Jolla, CA 92093, USA
  4.Department of Biology, San Francisco State University, San Francisco, CA 94132, USA
  5.The MOE Key Laboratory of Weak-Light Nonlinear Photonics, TEDA Applied Physical Institute and School of Physics, Nankai University, Tianjin 300457, China
- Author bio：
  
  Z Chen, Email: zhigang@sfsu.edu
- Funds：
- DOI：10.1038/lsa.2016.158
  CLC：
- Published：2016-12，
  
  Published Online：09 May 2016，
  
  Received：22 January 2016，
  
  Revised：28 April 2016，
  
  Accepted：03 May 2016
- Accepted：
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Bezryadina, A. S. et al. Optical disassembly of cellular clusters by tunable 'tug-of-war' tweezers. Light: Science & Applications, 5, e16158 (2016).
DOI：

Bezryadina, A. S. et al. Optical disassembly of cellular clusters by tunable 'tug-of-war' tweezers. Light: Science & Applications, 5, e16158 (2016). DOI： 10.1038/lsa.2016.158.

摘要

Abstract

Bacterial biofilms underlie many persistent infections

posing major hurdles in antibiotic treatment. Here we design and demonstrate 'tug-of-war' optical tweezers that can facilitate the assessment of cell–cell adhesion—a key contributing factor to biofilm development

thanks to the combined actions of optical scattering and gradient forces. With a customized optical landscape distinct from that of conventional tweezers

not only can such 'tug-of-war' tweezers stably trap and stretch a rod-shaped bacterium in the observing plane

but

more importantly

they can also impose a tunable lateral force that pulls apart cellular clusters without any tethering or mechanical movement. As a proof of principle

we examined a

Sinorhizobium meliloti

strain that forms robust biofilms and found that the strength of intercellular adhesion depends on the growth medium. This technique may herald new photonic tools for optical manipulation and biofilm study

as well as other biological applications.

关键词

Keywords

bacterial adhesionbiofilmoptical forcesoptical manipulationtug-of-war optical tweezers

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