Ultrafast laser-scanning time-stretch imaging at visible wavelengths

Jiang-Lai Wu; Yi-Qing Xu; Jing-Jiang Xu; Xiao-Ming Wei; Antony CS Chan; Anson HL Tang; Andy KS Lau; Bob MF Chung; Ho Cheung Shum; Edmund Y Lam; Kenneth KY Wong; Kevin K Tsia

doi:10.1038/lsa.2016.196

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Ultrafast laser-scanning time-stretch imaging at visible wavelengths

Original Articles

- Ultrafast laser-scanning time-stretch imaging at visible wavelengths
- Light: Science & Applications Vol. 6, Issue 1, (2017)
- Affiliations：
  
  1.Department of Electrical and Electronic Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong 999077, China
  2.Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA
  3.Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong 999077, China
- Author bio：
  
  KK Tsia, Email: tsia@hku.hk
- Funds：
- DOI：10.1038/lsa.2016.196
  CLC：
- Published：2017-03，
  
  Published Online：12 August 2016，
  
  Received：12 April 2016，
  
  Revised：04 August 2016，
  
  Accepted：11 August 2016
- Accepted：
Scan QR Code
Wu, J. L. et al. Ultrafast laser-scanning time-stretch imaging at visible wavelengths. Light: Science & Applications, 6, e16196 (2017).
DOI：

Wu, J. L. et al. Ultrafast laser-scanning time-stretch imaging at visible wavelengths. Light: Science & Applications, 6, e16196 (2017). DOI： 10.1038/lsa.2016.196.

摘要

Abstract

Optical time-stretch imaging enables the continuous capture of non-repetitive events in real time at a line-scan rate of tens of MHz—a distinct advantage for the ultrafast dynamics monitoring an

d high-throughput screening that are widely needed in biological microscopy. However

its potential is limited by the technical challenge of achieving significant pulse stretching (that is

high temporal dispersion) and low optical loss

which are the critical factors influencing imaging quality

in the visible spectrum demanded in many of these applications. We present a new pulse-stretching technique

termed free-space angular-chirp-enhanced delay (FACED)

with three distinguishing features absent in the prevailing dispersive-fiber-based implementations: (1) it generates substantial

reconfigurable temporal dispersion in free space (

1 ns nm

−1

) with low intrinsic loss (

6 dB) at visible wavelengths; (2) its wavelength-invariant pulse-stretching operation introduces a new paradigm in time-stretch imaging

which can now be implemented both with and without spectral encoding; and (3) pulse stretching in FACED inherently provides an ultrafast all-optical laser-beam scanning mechanism at a line-scan rate of tens of MHz. Using FACED

we demonstrate not only ultrafast laser-scanning time-stretch imaging with superior bright-field image quality compared with previous work but also

for the first time

MHz fluorescence and colorized time-stretch microscopy. Our results show that this technique could enable a wider scope of applications in high-speed and high-throughput biological microscopy that were once out of reach.

关键词

Keywords

optical time-stretch imagingpulse stretchingultrafast laser scanning

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