Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators

X Luís Deán-Ben; Gali Sela; Antonella Lauri; Moritz Kneipp; Vasilis Ntziachristos; Gil G Westmeyer; Shy Shoham; Daniel Razansky

doi:10.1038/lsa.2016.201

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Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators

Original Articles

- Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators
- Light: Science & Applications Vol. 5, Issue 4, (2016)
- Affiliations：
  
  1.Institute for Biological and Medical Imaging (IBMI), Helmholtz Center Munich, Neuherberg, Germany
  2.Institute of Developmental Genetics, Helmholtz Center Munich, Neuherberg, Germany
  3.Department of Medicine, Technical University of Munich, Munich, Germany
  4.Department of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel
- Author bio：
  
  GG Westmeyer, Email: gil.westmeyer@tum.de
  S Shoham, Email: shy@technion.ac.il
  D Razansky, Email: dr@tum.de
- Funds：
- DOI：10.1038/lsa.2016.201
  CLC：
- Published：2016-12，
  
  Published Online：15 August 2016，
  
  Received：21 March 2016，
  
  Revised：09 August 2016，
  
  Accepted：11 August 2016
- Accepted：
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Deán-Ben, X. L. et al. Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators. Light: Science & Applications, 5, e16201 (2016).
DOI：

Deán-Ben, X. L. et al. Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators. Light: Science & Applications, 5, e16201 (2016). DOI： 10.1038/lsa.2016.201.

摘要

Abstract

Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience.

We developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains. It can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm

with respective spatial resolution of 35–200 μm. Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate

for the first time

the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering brains. The newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics; in combination with optoacoustics' well-established capacity for resolving vascular hemodynamics

it could open new vistas in the study of neural activity and neurovascular coupling in health and disease.

关键词

Keywords

functional neuro-imaginggenetically encoded calcium indicatorshigh spatiotemporal resolutionlarge-scale brain activityoptoacoustic tomographyphotoacousticsreal-time imaging

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