Tomographic flow cytometry by digital holography

Francesco Merola; Pasquale Memmolo; Lisa Miccio; Roberto Savoia; Martina Mugnano; Angelo Fontana; Giuliana D'Ippolito; Angela Sardo; Achille Iolascon; Antonella Gambale; Pietro Ferraro

doi:10.1038/lsa.2016.241

Go to Nature Website

Your Location：

Home >

Browse articles >

Tomographic flow cytometry by digital holography

Original Articles

- Tomographic flow cytometry by digital holography
- Light: Science & Applications Vol. 6, Issue 2, (2017)
- Affiliations：
  
  1.CNR-ISASI, Istituto di Scienze Applicate e Sistemi Intelligenti 'E. Caianiello', CNR—Consiglio Nazionale delle Ricerche, Pozzuoli 80078, Italy
  2.CNR-ICB, Istituto di Chimica Biomolecolare, Pozzuoli 80078, Italy
  3.Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico Ⅱ & CEINGE—Advanced Biotechnologies, Napoli 80145, Italy
- Author bio：
  
  P Ferraro, Email: pietro.ferraro@cnr.it
- Funds：
- DOI：10.1038/lsa.2016.241
  CLC：
- Published：2017-06，
  
  Published Online：17 October 2016，
  
  Received：04 May 2016，
  
  Revised：03 October 2016，
  
  Accepted：10 October 2016
- Accepted：
Scan QR Code
Merola, F. et al. Tomographic flow cytometry by digital holography. Light: Science & Applications, 6, e16241 (2017).
DOI：

Merola, F. et al. Tomographic flow cytometry by digital holography. Light: Science & Applications, 6, e16241 (2017). DOI： 10.1038/lsa.2016.241.

摘要

Abstract

High-throughput single-cell analysis is a challenging task. Label-free tomographic phase microscopy is an excellent candidate to perform this task. However

in-line tomography is very difficult to implement in practice because it requires a complex set-up for rotating the sample and examining the cell along several directions. We demonstrate that by exploiting the random rolling of cells while they are flowing along a microfluidic channel

it is possible to obtain in-line phase-contrast tomography

if smart strategies for wavefront analysis are adopted. In fact

surprisingly

a priori

knowledge of the three-dimensional position and orientation of rotating cells is no longer needed because this information can be completely retrieved through digital holography wavefront numerical analysis. This approach makes continuous-flow cytotomography suitable for practical operation in real-world

single-cell analysis and with a substantial simplification of the optical system; that is

no mechanical scanning or multi-direction probing is required. A demonstration is given for two completely different classes of biosamples: red blood cells and diatom algae. An accurate characterization of both types of cells is reported

despite their very different nature and material content

thus showing that the proposed method can be extended by adopting two alternate strategies of wavefront analysis to many classes of cells.

关键词

Keywords

microfluidicsred blood cellstomographic microscopywavefront analysis

references

Mertz J. Optical sectioning microscopy with planar or structured illumination.Nat Methods2011;8: 811–819..

Huang D, Swanson EA, Lin CP, Schuman JS, Stinson WGet al. Optical coherence tomography.Science1991;254: 1178–1181..

Yi J, Liu WZ, Chen SY, Backman V, Sheibani Net al. Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation.Light Sci Appl2015;4: e334 doi:10.1038/lsa.2015.107..

Choi W, Fang-Yen C, Badizadegan K, Oh S, Lue Net al. Tomographic phase microscopy.Nat Methods2007;4: 717–719..

Kim K, Kim KS, Park H, Ye JC, Park YK. Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography.Opt Express2013;21: 32269–32278..

Charrière F, Marian A, Montfort F, Kuehn J, Colomb Tet al. Cell refractiveindex tomography by digital holographic microscopy.Opt Lett2006;31: 178–180..

Kim T, Zhou RJ, Mir M, Babacan SD, Carney PSet al. White-light diffraction tomography of unlabelled live cells.Nat Photon2014;8: 256–263..

Mir M, Tangella K, Popescu G. Blood testing at the single cell level using quantitative phase and amplitude microscopy.Biomed Opt Express2011;2: 3259–3266..

Kim Y, Shim H, Kim K, Park H, Jang Set al. Profiling individual human red blood cells using common-path diffraction optical tomography.Sci Rep2014;4: 6659..

Sung Y, Lue N, Hamza B, Martel J, Irimia Det al. Three-dimensional holographic refractive-index measurement of continuously flowing cells in a microfluidic channel.Phys Rev Appl2014;1: 014002..

Yoon J, Kim K, Park H, Choi C, Jang Set al. Label-free characterization of white blood cells by measuring 3D refractive index maps.Biomed Opt Express2015;6: 3865–3875..

Bishara W, Zhu HY, Ozcan A. Holographic opto-fluidic microscopy.Opt Express2010;18: 27499–27510..

Isikman SO, Bishara W, Zhu HY, Ozcan A. Optofluidic tomography on a chip.Appl Phys Lett2011;98: 161109..

Isikman SO, Bishara W, Mavandadi S, Yu FW, Feng Set al. Lens-free optical tomographic microscope with a large imaging volume on a chip.Proc Natl Acad Sci USA2011;108: 7296–7301..

Kamilov US, Papadopoulos IN, Shoreh MH, Goy A, Vonesch Cet al. Learning approach to optical tomography.Optica2015;2: 517–522..

Psaltis D, Quake SR, Yang C. Developing optofluidic technology through the fusion of microfluidics and optics.Nature2006;442: 381–386..

Pégard NC, Toth ML, Driscoll M, Fleischer JW. Flow-scanning optical tomography.Lab Chip2014;14: 4447–4450..

Padgett M, Di Leonardo R. Holographic optical tweezers and their relevance to lab on chip devices.Lab Chip2011;11: 1196–1205..

Merola F, Miccio L, Memmolo P, Di Caprio G, Galli Aet al. Digital holography as a method for 3D imaging and estimating the biovolume of motile cells.Lab Chip2013;13: 4512–4516..

Memmolo P, Miccio L, Merola F, Gennari O, Netti PAet al. 3D morphometry of red blood cells by digital holography.Cytometry A2014;85: 1030–1036..

Habaza M, Gilboa B, Roichman Y, Shaked NT. Tomographic phase microscopy with 180° rotation of live cells in suspension by holographic optical tweezers.Opt Lett2015;40: 1881–1884..

Heng X, Erickson D, Baugh LR, Yaqoob Z, Sternberg PWet al. Optofluidic microscopy—a method for implementing a high resolution optical microscope on a chip.Lab Chip2006;6: 1274–1276..

Miccio L, Memmolo P, Merola F, Netti PA, Ferraro P. Red blood cell as an adaptive optofluidic microlens.Nat Commun2015;6: 6502..

Elgsaeter A, Stokke BT, Mikkelsen A, Branton D. The molecular basis of erythrocyte shape.Science1986;234: 1217–1223..

Ford J. Red blood cell morphology.Int J Lab Hematol2013;35: 351–357..

Bedoshvili YD, Popkova TP, Likhoshway YV. Chloroplast structure of diatoms of different classes.Cell Tiss Biol2009;3: 297–310..

Wang LP, Zheng BH. Toxic effects of fluoranthene and copper on marine diatomPhaeodactylum tricornutum.J Environ Sci2008;20: 1363–1372..

Arpali SA, Arpali C, Coskun AF, Chiang HH, Ozcan A. High-throughput screening of large volumes of whole blood using structured illumination and fluorescent on-chip imaging.Lab Chip2012;12: 4968–4971..

Uspal WE, Burak Eral H, Doyle PS. Engineering particle trajectories in microfluidic flows using particle shape.Nat Commun2013;4: 2666..

D'Avino G, Maffettone PL. Particledynamics in viscoelastic liquids.J Non-Newtonian Fluid Mech2015;215: 80–104..

Amini H, Lee W, Di Carlo D. Inertial microfluidic physics.Lab Chip2014;14: 2739–2761..

Pham HV, Bhaduri B, Tangella K, Best-Popescu C, Popescu G. Real time blood testing using quantitative phase imaging.PLoS One2013;8: e55676..

Merola F, Memmolo P, Miccio L, Bianco V, Paturzo Met al. Diagnostic tools for lab-on-chip applications based on coherent imaging microscopy.Proc IEEE2015;103: 192–204..

Cotte Y, Toy MF, Depeursinge C. Beyond the lateral resolution limit by phase imaging.J Biomed Opt2011;16: 106007..

Kim MK, Yu LF, Mann CJ. Interference techniques in digital holography.J Opt A Pure Appl Opt2006;8: S518..

Villone MM, D'Avino G, Hulsen MA, Maffettone PL. Dynamics of prolate spheroidal elastic particles in confined shear flow.Phys Rev E2015;92: 062303..

Sharpe J, Ahlgren U, Perry P, Hill B, Ross Aet al. Optical projection tomography as a tool for 3D microscopy and gene expression studies.Science2002;296: 541–545..

Dardikman G, Habaza M, Waller L, Shaked NT. Video-rate processing in tomographic phase microscopy of biological cells using CUDA.Opt Express2016;24: 11839–11854..

Memmolo P, Miccio L, Paturzo M, Di Caprio G, Coppola Get al. Recent advances in holographic 3D particle tracking.Adv Opt Photon2015;7: 713–755..

Memmolo P, Iannone M, Ventre M, Netti PA, Finizio Aet al. On the holographic 3D tracking ofin vitrocells characterized by a highly-morphological change.Opt Express2012;20: 28485–28493..

Memmolo P, Distante C, Paturzo M, Finizio A, Ferraro Pet al. Automatic focusing in digital holography and its application to stretched holograms.Opt Lett2011;36: 1945–1947..

De Falco L, Silvestri L, Kannengiesser C, Morán E, Oudin Cet al. Functional and clinical impact of novel TMPRSS6 variants in iron-refractory iron-deficiency anemia patients and genotype-phenotype studies.Hum Mutat2014;35: 1321–1329..

Sessa R, Puzone S, Ammirabile M, Piscopo C, Pagano Let al. Identification and molecular characterization of the—CAMPANIA deletion, a novel a°-thalassemic defect, in two unrelated Italian families.Am J Hematol2010;85: 143–144..

Origa R, Moi P, Galanello R, Cao A. Alpha-thalassemia. In: Pagon RAet al. editors.GeneReviews. Seattle, WA: University of Washington; 2013..

Fedosov DA, Peltomäki M, Gompper G. Deformation and dynamics of red blood cells in flow through cylindrical microchannels.Soft Matter2014;10: 4258–4267..

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰