Correction-free remotely scanned two-photon <italic style="font-style: italic">in vivo</italic> mouse retinal imaging

Adi Schejter Bar-Noam; Nairouz Farah; Shy Shoham

doi:10.1038/lsa.2016.7

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Correction-free remotely scanned two-photon in vivo mouse retinal imaging

Original Articles

- Correction-free remotely scanned two-photon in vivo mouse retinal imaging
- Light: Science & Applications Vol. 5, Issue 1, (2016)
- Affiliations：
  
  Faculty of Biomedical Engineering, Technion – Israel Institute of Technology, Kiryat HaTechnion, Haifa 32000, Israel
- Author bio：
  
  S Shoham, Email: shy@technion.ac.il
- Funds：
- DOI：10.1038/lsa.2016.7
  CLC：
- Published：2016-03，
  
  Published Online：23 August 2015，
  
  Received：07 March 2015，
  
  Revised：14 August 2015，
  
  Accepted：17 August 2015
- Accepted：
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Bar-Noam, A. S., Farah, N. & Shoham, S. Correction-free remotely scanned two-photon in vivo mouse retinal imaging. Light: Science & Applications, 5, e16007 (2016).
DOI：

Bar-Noam, A. S., Farah, N. & Shoham, S. Correction-free remotely scanned two-photon in vivo mouse retinal imaging. Light: Science & Applications, 5, e16007 (2016). DOI： 10.1038/lsa.2016.7.

摘要

Abstract

Non-invasive fluorescence retinal imaging in small animals is an important requirement for an array of translational vision applications. The

in vivo

two-photon imaging of the mouse retina may enable the long-term investigation of the structure and function of healthy and diseased retinal tissue. However

to date

this has only been possible using relatively c

omplex adaptive-optics systems. Here

the optical modeling of the murine eye and of the imaging system is used to achieve correction-free two-photon microscopy through the pupil of a mouse eye to yield high-quality

optically sectioned fundus images. By remotely scanning the focus using an electronically tunable lens

high-resolution three-dimensional fluorescein angiograms and cellular-scale images are acquired

thus introducing a correction-free baseline performance level for two-photon

in vivo

retinal imaging. Moreover

the system enables functional calcium imaging of repeated retinal responses to light stimulation using the genetically encoded indicator

GCaMP6s. These results and the simplicity of the new add-on optics are an important step toward several structural

functional

and multimodal imaging applications that will benefit from the tight optical sectioning and the use of near-infrared light.

关键词

Keywords

angiographyeye modelfunctional calcium imagingin vivooptogeneticstwo-photon microscopy

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⁰

Correction-free remotely scanned two-photon in vivo mouse retinal imaging

DOI：10.1038/lsa.2016.7

摘要

Abstract

关键词

Keywords

references