Long-term <italic style="font-style: italic">in vivo</italic> single-cell lineage tracing of deep structures using three-photon activation

Isil Tekeli; Isabelle Aujard; Xavier Trepat; Ludovic Jullien; Angel Raya; Dobryna Zalvidea

doi:10.1038/lsa.2016.84

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Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation

Original Articles

- Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation
- Light: Science & Applications Vol. 5, Issue 3, (2016)
- Affiliations：
  
  1.Center of Regenerative Medicine in Barcelona (CMRB), Barcelona Biomedical Research Park, Dr Aiguader 88, 08003 Barcelona, Spain
  2.Control of Stem Cell Potency Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona Science Park, Baldiri Reixac 15-21, 08028 Barcelona, Spain
  3.École Normale Supérieure—PSL Research University, Department of Chemistry, 24 rue Lhomond, F-75005 Paris, France
  4.Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005 Paris, France
  5.CNRS, UMR 8640 PASTEUR, F-75005 Paris, France
  6.Integrative Cell and Tissue Dynamics Group, Institute of Bioengineering of Catalonia (IBEC), 08028 Barcelona, Spain
  7.Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain
  8.Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain
- Author bio：
  
  A Raya, Email: araya@cmrb.eu
  D Zalvidea, Email: dzalvidea@ibecbarcelona.eu
- Funds：
- DOI：10.1038/lsa.2016.84
  CLC：
- Published：2016-09，
  
  Published Online：29 January 2016，
  
  Received：09 July 2015，
  
  Revised：25 January 2016，
  
  Accepted：27 January 2016
- Accepted：
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Tekeli, I. et al. Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation. Light: Science & Applications, 5, e16084 (2016).
DOI：

Tekeli, I. et al. Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation. Light: Science & Applications, 5, e16084 (2016). DOI： 10.1038/lsa.2016.84.

摘要

Abstract

Genetic labeling techniques allow for n

oninvasive lineage tracing of cells

in vivo

. Two-photon inducible activators provide spatial resolution for superficial cells

but labeling cells located deep within tissues is precluded by scattering of the far-red illumination required for two-photon photolysis. Three-photon illumination has been shown to overcome the limitations of two-photon microscopy for

in vivo

imaging of deep structures

but whether it can be used for photoactivation remains to be tested. Here we show

both theoretically and experimentally

that three-photon illumination overcomes scattering problems by combining longer wavelength excitation with high uncaging three-photon cross-section molecules. We prospectively labeled heart muscle cells in zebrafish embryos and found permanent labeling in their progeny in adult animals with negligible tissue damage. This technique allows for a noninvasive genetic manipulation

in vivo

with spatial

temporal and cell-type specificity

and may have wide applicability in experimental biology.

关键词

Keywords

multi-photon microscopyphotoactivationthree-photon microscopyzebrafish

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Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation

DOI：10.1038/lsa.2016.84

摘要

Abstract

关键词

Keywords

references