Luminescent gold nanocluster-based sensing platform for accurate H<sub>2</sub>S detection <italic style="font-style: italic">in vitro</italic> and <italic style="font-style: italic">in vivo</italic> with improved anti-interference

Qi Yu; Pengli Gao; Kenneth Yin Zhang; Xiao Tong; Huiran Yang; Shujuan Liu; Jing Du; Qiang Zhao; Wei Huang

doi:10.1038/lsa.2017.107

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Luminescent gold nanocluster-based sensing platform for accurate H₂S detection in vitro and in vivo with improved anti-interference

Original Articles

- Luminescent gold nanocluster-based sensing platform for accurate H₂S detection in vitro and in vivo with improved anti-interference
- Light: Science & Applications Vol. 6, Issue 4, (2017)
- Affiliations：
  
  1.Key Laboratory for Organic Electronics and Information Displays and Institute of Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing 210023, China
  2.Key Laboratory of Flexible Electronics and Institute of Advanced Materials, Jiangsu National Synergetic Innovation Center for Advanced Materials, Nanjing Tech University, Nanjing 211816, China
- Author bio：
  
  Q Zhao, Email: iamqzhao@njupt.edu.cn
  W Huang, Email: wei-huang@njtech.edu.cn
- Funds：
- DOI：10.1038/lsa.2017.107
  CLC：
- Published：2017-12，
  
  Published Online：26 June 2017，
  
  Received：09 September 2016，
  
  Revised：22 June 2017，
  
  Accepted：23 June 2017
- Accepted：
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Yu, Q. et al. Luminescent gold nanocluster-based sensing platform for accurate H₂S detection in vitro and in vivo with improved anti-interference. Light: Science & Applications, 6, e17107 (2017).
DOI：

Yu, Q. et al. Luminescent gold nanocluster-based sensing platform for accurate H₂S detection in vitro and in vivo with improved anti-interference. Light: Science & Applications, 6, e17107 (2017). DOI： 10.1038/lsa.2017.107.

摘要

Abstract

Gold nanoclusters (Au NCs) are promising luminescent nanomaterials due to their outstanding optical properties. However

their relatively low quantum yields and environment-dependent photoluminescence properties have limited their biological applications. To address these problems

we developed a novel strategy to prepare chitosan oligosaccharide lactate (Chi)-functionalized Au NCs (Au NCs@Chi)

which exhibited emission with enhanced quantum yield and elongated emission lifetime as compared to the Au NCs

as well as exhibited environment-independent photoluminescence properties. In addition

utilizing the free amino groups of Chi onto Au NCs@Chi

we designed a FRET-based sensing platform for the detection of hydrogen sulfide (H

S). The Au

NCs and the specific H

S-sensitive merocyanine compound were respectively employed as an energy donor and acceptor in the platform. The addition of H

S induced changes in the emission profile and luminescence lifetime of the platform with high sensitivity and selectivity. Utilization of the platform was demonstrated to detect exogenous and endogenous H

in vitro

and

in vivo

through wavelength-ratiometric and time-resolved luminescence imaging (TLI). Compared to previously reported luminescent molecules

the platform was less affected by experimental conditions and showed minimized autofluorescence interference and improved accuracy of detection.

关键词

Keywords

bioimaginggold nanoclustershydrogen sulfideluminescent sensorsphotoluminescence

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Luminescent gold nanocluster-based sensing platform for accurate H2S detection in vitro and in vivo with improved anti-interference

DOI：10.1038/lsa.2017.107

摘要

Abstract

关键词

Keywords

references

Luminescent gold nanocluster-based sensing platform for accurate H₂S detection in vitro and in vivo with improved anti-interference