Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques

Sara Mattana; Maurizio Mattarelli; Lorena Urbanelli; Krizia Sagini; Carla Emiliani; Mauro Dalla Serra; Daniele Fioretto; Silvia Caponi

doi:10.1038/lsa.2017.139

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Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques

Original Articles

- Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques
- Light: Science & Applications Vol. 7, Issue 1, (2018)
- Affiliations：
  
  1.Department of Physics and Geology, University of Perugia, Perugia I-06123, Italy
  2.Laboratory of Biochemistry and Molecular Biology, Department of Chemistry, Biology and Biotechnology, University of Perugia, via del Giochetto, Perugia I-06123, Italy
  3.CEMIN-Center of Excellence for Innovative Nanostructured Material, Perugia I-06123, Italy
  4.Istituto di Biofisica CNR (IBF-CNR), Unità di Trento, and FBK, Via Sommarive 18, Trento 38123, Italy
  5.Istituto Officina dei Materiali del CNR (CNR-IOM)—Unità di Perugia, c/o Department of Physics and Geology, University of Perugia, Perugia I-06123, Italy
- Author bio：
  
  S Caponi, Email: silvia.caponi@cnr.it
- Funds：
- DOI：10.1038/lsa.2017.139
  CLC：
- Published：2018，
  
  Published Online：09 February 2018，
  
  Received：10 May 2017，
  
  Revised：23 September 2017，
  
  Accepted：08 October 2017
- Accepted：
Scan QR Code
Mattana, S. et al. Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques. Light: Science & Applications, 1, 17139 (2018).
DOI：

Mattana, S. et al. Non-contact mechanical and chemical analysis of single living cells by microspectroscopic techniques. Light: Science & Applications, 1, 17139 (2018). DOI： 10.1038/lsa.2017.139.

摘要

Abstract

Innovative label-free microspectroscopy

which can simultaneously collect Brillouin and Raman signals

is used to characterize the viscoelastic properties and chemical composition of living cells with sub-micrometric resolution. The unprecedented statistical accuracy of the data combined with the high-frequency resolution and the high contrast of the recently built experimental setup permits the study of single living cells immersed in their buffer solution by contactless measurements. The Brillouin signal is deconvoluted in the buffer and the cell components

thereby revealing the mechanical heterogeneity inside the cell. In particular

a 20% increase is observed in the elastic modulus passing from the plasmatic membrane to the nucleus as distinguished by comparison with the Raman spectroscopic marker. Brillouin line shape analysis is even more relevant for the comparison of cells under physiological and pathological conditions. Following oncogene expression

cells show an overall reduction in the elastic modulus (15%) and apparent viscosity (50%). In a proof-of-principle experiment

the ability of this spectroscopic technique to characterize subcellular compartments and distinguish cell status was successfully tested. The results strongly support the future application of this technique for fundamental issues in the biomedical field.

关键词

Keywords

living single cell analysismechanical propertiesRaman and Brillouin spectroscopy

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