Skull optical clearing window for <italic style="font-style: italic">in vivo</italic> imaging of the mouse cortex at synaptic resolution

Yan-Jie Zhao; Ting-Ting Yu; Chao Zhang; Zhao Li; Qing-Ming Luo; Tong-Hui Xu; Dan Zhu

doi:10.1038/lsa.2017.153

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Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution

Original Articles

- Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution
- Light: Science & Applications Vol. 7, Issue 1, (2018)
- Affiliations：
  
  1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China
  2.MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China
- Author bio：
  
  D Zhu, Email: dawnzh@mail.hust.edu.cn
  TH Xu, Email: xutonghui@hust.edu.cn
- Funds：
- DOI：10.1038/lsa.2017.153
  CLC：
- Published：2018，
  
  Published Online：23 February 2018，
  
  Received：03 October 2017，
  
  Revised：27 October 2017，
  
  Accepted：27 October 2017
- Accepted：
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Zhao, Y. J. et al. Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution. Light: Science & Applications, 1, 17153 (2018).
DOI：

Zhao, Y. J. et al. Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution. Light: Science & Applications, 1, 17153 (2018). DOI： 10.1038/lsa.2017.153.

摘要

Abstract

Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here

we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy

this technique allowed us to repeatedly image neurons

microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety

this method holds great promise for application in neuroscience research.

关键词

Keywords

cranial windowdendritic spinesoptical clearingsynaptic resolutiontwo-photon imaging

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Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution

DOI：10.1038/lsa.2017.153

摘要

Abstract

关键词

Keywords

references