Nanoplasmonic mid-infrared biosensor for <italic style="font-style: italic">in vitro</italic> protein secondary structure detection

Dordaneh Etezadi; John B Warner Ⅳ; Francesco S Ruggeri; Giovanni Dietler; Hilal A Lashuel; Hatice Altug

doi:10.1038/lsa.2017.29

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Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection

Original Articles

- Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection
- Light: Science & Applications Vol. 6, Issue 3, (2017)
- Affiliations：
  
  1.Bionanophotonic Systems Laboratory, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne CH-1015, Switzerland
  2.Laboratory of Molecular Neurobiology and Neuroproteomics, EPFL, Lausanne CH-1015, Switzerland
  3.Institute of Physics, Laboratory of the Physics of Living Matter, EPFL, Lausanne CH-1015, Switzerland
  4.Department of Chemistry, Lensfield road, University of Cambridge, Cambridge CB21EW, UK
- Author bio：
  
  H Altug, Email: hatice.altug@epfl.ch
- Funds：
- DOI：10.1038/lsa.2017.29
  CLC：
- Published：2017-09，
  
  Published Online：27 February 2017，
  
  Received：13 July 2016，
  
  Revised：17 February 2017，
  
  Accepted：23 February 2017
- Accepted：
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Etezadi, D. et al. Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection. Light: Science & Applications, 6, e17029 (2017).
DOI：

Etezadi, D. et al. Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection. Light: Science & Applications, 6, e17029 (2017). DOI： 10.1038/lsa.2017.29.

摘要

Abstract

Plasmonic nanoantennas offer new applications in mid-infrared (mid-IR) absorption spectroscopy with ultrasensitive detection of structural signatures of biomolecules

such as proteins

due to their strong resonant near-fields. The amide Ⅰ fingerprint of a protein contains conformational information that is greatly important for understanding

its function in health and disease. Here

we introduce a non-invasive

label-free mid-IR nanoantenna-array sensor for secondary structure identification of nanometer-thin protein layers in aqueous solution by resolving the content of plasmonically enhanced amide Ⅰ signatures. We successfully detect random coil to cross β-sheet conformational changes associated with α-synuclein protein aggregation

a detrimental process in many neurodegenerative disorders. Notably

our experimental results demonstrate high conformational sensitivity by differentiating subtle secondary-structural variations in a native β-sheet protein monolayer from those of cross β-sheets

which are characteristic of pathological aggregates. Our nanoplasmonic biosensor is a highly promising and versatile tool for

in vitro

structural analysis of thin protein layers.

关键词

Keywords

label-free biosensingnanoantennasplasmonicsprotein secondary structuresurface-enhanced infrared absorption spectroscopy

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⁰

Nanoplasmonic mid-infrared biosensor for in vitro protein secondary structure detection

DOI：10.1038/lsa.2017.29

摘要

Abstract

关键词

Keywords

references