Endowing a plain fluidic chip with micro-optics: a holographic microscope slide

Vittorio Bianco; Biagio Mandracchia; Valentina Marchesano; Vito Pagliarulo; Federico Olivieri; Sara Coppola; Melania Paturzo; Pietro Ferraro

doi:10.1038/lsa.2017.55

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Endowing a plain fluidic chip with micro-optics: a holographic microscope slide

Original Articles

- Endowing a plain fluidic chip with micro-optics: a holographic microscope slide
- Light: Science & Applications Vol. 6, Issue 3, (2017)
- Affiliations：
  
  1.Institute of Applied Sciences and Intelligent Systems—Italian National Research Council (ISASI-CNR), Via Campi Flegrei 34, Pozzuoli, Napoli 80078, Italy
  2.Università degli Studi di Napoli 'Federico Ⅱ', P.le Tecchio 80, Napoli 80100, Italy
- Author bio：
  
  V Bianco, Email: v.bianco@isasi.cnr.it
- Funds：
- DOI：10.1038/lsa.2017.55
  CLC：
- Published：2017-09，
  
  Published Online：29 March 2017，
  
  Received：07 November 2016，
  
  Revised：22 March 2017，
  
  Accepted：23 March 2017
- Accepted：
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Bianco, V. et al. Endowing a plain fluidic chip with micro-optics: a holographic microscope slide. Light: Science & Applications, 6, e17055 (2017).
DOI：

Bianco, V. et al. Endowing a plain fluidic chip with micro-optics: a holographic microscope slide. Light: Science & Applications, 6, e17055 (2017). DOI： 10.1038/lsa.2017.55.

摘要

Abstract

Lab-on-a-Chip (LoC) devices are extremely promising in that they enable diagnostic functions at the point-of-care. Within this scope

an important goal is to design imaging schemes that can be used out of the laboratory. In this paper

we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup. Instead

a commercial off-the-shelf plastic chip is engineered and functionalized with this aim. The microfluidic chip is endowed with micro-optics

that is

a diffraction grating and polymeric lenses

to build an interferometer directly on the chip

avoiding the need for a reference arm and external bulky optical components. Thanks to the single-beam scheme

the system is completely integrated and robust against vibrations

sharing the useful features of any common path interferometer. Hence

it becomes possible to bring holographic functionalities out of the lab

moving complexity from the external optical apparatus to the chip itself. Label-free imaging and quantitative phase contrast mapping of live samples are demonstrated

along with flexible refocusing capabilities. Thus

a liquid volume can be analyzed in one single shot with no need for mechanical scanning systems.

关键词

Keywords

diffraction gratingsdigital holographyLab-on-a-Chipmicro-opticsoptical devicespolymer printing

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