Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter

Linli Shi; Ying Jiang; Fernando R. Fernandez; Guo Chen; Lu Lan; Heng-Ye Man; John A. White; Ji-Xin Cheng; Chen Yang

doi:10.1038/s41377-021-00580-z

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Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter

Articles

- Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter
- Light: Science & Applications Vol. 10, Issue 8, Pages: 1494-1506(2021)
- Affiliations：
  
  1.Department of Chemistry, Boston University, 580 Commonwealth Avenue, Boston, MA, 02215, USA
  2.Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA, 02215, USA
  3.Center for Systems Neuroscience, Boston University, 610 Commonwealth Ave, Boston, MA, 02215, USA
  4.Neurophotonics Center, Photonics Center, Boston University, 8 St. Mary's Street, Boston, MA, 02215, USA
  5.Department of Electrical and Computer Engineering, 8 St. Mary's Street, Boston, MA, 02215, USA
  6.Department of Biology, Boston University, 5 Cummington Mall, Boston, MA, 02215, USA
- Author bio：
  
  Ji-Xin Cheng (jxcheng@bu.edu)
  Chen Yang(cheyang@bu.edu)
- Funds：
- DOI：10.1038/s41377-021-00580-z
  CLC：
- Published：31 August 2021，
  
  Published Online：14 July 2021，
  
  Received：03 February 2021，
  
  Revised：09 June 2021，
  
  Accepted：21 June 2021
- Accepted：
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Shi, L. L. et al. Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter. Light: Science & Applications, 10, 1494-1506 (2021).
DOI：

Shi, L. L. et al. Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter. Light: Science & Applications, 10, 1494-1506 (2021). DOI： 10.1038/s41377-021-00580-z.

摘要

Abstract

Neuromodulation at high spatial resolution poses great significance in advancing fundamental knowledge in the field of neuroscience and offering novel clinical treatments. Here

we developed a tapered fiber optoacoustic emitter (TFOE) generating an ultrasound field with a high spatial precision of 39.6 µm

enabling optoacoustic activation of single neurons or subcellular structures

such as axons and dendrites. Temporally

a single acoustic pulse of sub-microsecond converted by the TFOE from a single laser pulse of 3 ns is shown as the shortest acoustic stimuli so far for successful neuron activation. The precise ultrasound generated by the TFOE enabled the integration of the optoacoustic stimulation with highly stable patch-clamp recording on single neurons. Direct measurements of the electrical response of single neurons to acoustic stimulation

which is difficult for conventional ultrasound stimulation

have been demonstrated. By coupling TFOE with ex vivo brain slice electrophysiology

we unveil cell-type-specific responses of excitatory and inhibitory neurons to acoustic stimulation. These results demonstrate that TFOE is a non-genetic single-cell and sub-cellular modulation technology

which could shed new insights into the mechanism of ultrasound neurostimulation.

关键词

Keywords

references

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