
1.Department of Biomedical Engineering, University of California Davis, Davis, CA, 95616, USA
2.Department of Pathology and Laboratory Medicine, University of California Davis Medical Center, Sacramento, CA, 95817, USA
3.Department of Ophthalmology and Vision Science, School of Medicine, University of California Davis, Sacramento, CA, 95817, USA
4.Department of Ophthalmology, NYU Langone Health, New York, NY, 10017, USA
5.Department of Radiology, NYU Langone Health, New York, NY, 10016, USA
6.Tech4Health Institute, NYU Langone Health, New York, NY, 10010, USA
Vivek J. Srinivasan (vjsriniv@ucdavis.edu)
Published:31 August 2021,
Published Online:14 July 2021,
Received:01 February 2021,
Revised:25 June 2021,
Accepted:29 June 2021
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Zhu, J. et al. 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain. Light: Science & Applications, 10, 1507-1519 (2021).
Zhu, J. et al. 1700 nm optical coherence microscopy enables minimally invasive, label-free, in vivo optical biopsy deep in the mouse brain. Light: Science & Applications, 10, 1507-1519 (2021). DOI: 10.1038/s41377-021-00586-7.
In vivo
minimally invasive microscopy in deep cortical and sub-cortical regions of the mouse brain has been challenging. To address this challenge
we present an in vivo high numerical aperture optical coherence microscopy (OCM) approach that fully utilizes the water absorption window around 1700 nm
where ballistic attenuation in the brain is minimized. Key issues
including detector noise
excess light source noise
chromatic dispersion
and the resolution-speckle tradeoff
are analyzed and optimized. Imaging through a thinned-skull preparation that preserves intracranial space
we present volumetric imaging of cytoarchitecture and myeloarchitecture across the entire depth of the mouse neocortex
and some sub-cortical regions. In an Alzheimer's disease model
we report that findings in superficial and deep cortical layers diverge
highlighting the importance of deep optical biopsy. Compared to other microscopic techniques
our 1700 nm OCM approach achieves a unique combination of intrinsic contrast
minimal invasiveness
and high resolution for deep brain imaging.
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