Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

Nadja Gustavsson; Agnes Paulus; Isak Martinsson; Anders Engdahl; Kadda Medjoubi; Konstantin Klementiev; Andrea Somogyi; Tomas Deierborg; Ferenc Borondics; Gunnar K. Gouras; Oxana Klementieva

doi:10.1038/s41377-021-00590-x

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Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons

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- Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons
- Light: Science & Applications Vol. 10, Issue 8, Pages: 1536-1545(2021)
- Affiliations：
  
  1.Medical Microspectroscopy, Department of Experimental Medical Science, Lund University, 22180, Lund, Sweden
  2.Experimental Neuroinflammation Lab, Department of Experimental Medical Science, Lund University, 22180, Lund, Sweden
  3.Experimental Dementia Research, Department of Experimental Medical Science, Lund University, 22180, Lund, Sweden
  4.Synchrotron SOLEIL, L'Orme des Merisiers, 91192, Gif Sur Yvette Cedex, France
  5.MAX IV Laboratory, 22100, Lund, Sweden
  6.Lund Institute for advanced Neutron and X-ray Science (LINXS), 223 70, Lund, Sweden
- Author bio：
  
  Oxana Klementieva (oxana.klementieva@med.lu.se)
- Funds：
- DOI：10.1038/s41377-021-00590-x
  CLC：
- Published：31 August 2021，
  
  Published Online：22 July 2021，
  
  Received：23 December 2020，
  
  Revised：20 June 2021，
  
  Accepted：05 July 2021
- Accepted：
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Gustavsson, N. et al. Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons. Light: Science & Applications, 10, 1536-1545 (2021).
DOI：

Gustavsson, N. et al. Correlative optical photothermal infrared and X-ray fluorescence for chemical imaging of trace elements and relevant molecular structures directly in neurons. Light: Science & Applications, 10, 1536-1545 (2021). DOI： 10.1038/s41377-021-00590-x.

摘要

Abstract

Alzheimer's disease (AD) is the most common cause of dementia

costing about 1% of the global economy. Failures of clinical trials targeting amyloid-β protein (Aβ)

a key trigger of AD

have been explained by drug inefficiency regardless of the mechanisms of amyloid neurotoxicity

which are very difficult to address by available technologies. Here

we combine two imaging modalities that stand at opposite ends of the electromagnetic spectrum

and therefore

can be used as complementary tools to assess structural and chemical information directly in a single neuron. Combining label-free super-resolution microspectroscopy for sub-cellular imaging based on novel optical photothermal infrared (O-PTIR) and synchrotron-based X-ray fluorescence (S-XRF) nano-imaging techniques

we capture elemental distribution and fibrillary forms of amyloid-β proteins in the same neurons at an unprecedented resolution. Our results reveal that in primary AD-like neurons

iron clusters co-localize with elevated amyloid β-sheet structures and oxidized lipids. Overall

our O-PTIR/S-XRF results motivate using high-resolution multimodal microspectroscopic approaches to understand the role of molecular structures and trace elements within a single neuronal cell.

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references

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