Label-free mid-infrared photothermal live-cell imaging beyond video rate

Genki Ishigane; Keiichiro Toda; Miu Tamamitsu; Hiroyuki Shimada; Venkata Ramaiah Badarla; Takuro Ideguchi

doi:10.1038/s41377-023-01214-2

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Label-free mid-infrared photothermal live-cell imaging beyond video rate

Original Articles

- Label-free mid-infrared photothermal live-cell imaging beyond video rate
- Light: Science & Applications Vol. 12, Issue 8, Pages: 1661-1674(2023)
- Affiliations：
  
  1.Department of Physics, The University of Tokyo, Tokyo, Japan
  2.Institute for Photon Science and Technology, The University of Tokyo, Tokyo, Japan
- Author bio：
  
  Takuro Ideguchi (ideguchi@ipst.s.u-tokyo.ac.jp)
- Funds：
- DOI：10.1038/s41377-023-01214-2
  CLC：
- Published：31 August 2023，
  
  Published Online：19 July 2023，
  
  Received：16 March 2023，
  
  Revised：14 June 2023，
  
  Accepted：21 June 2023
- Accepted：
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Ishigane, G. et al. Label-free mid-infrared photothermal live-cell imaging beyond video rate. Light: Science & Applications, 12, 1661-1674 (2023).
DOI：

Ishigane, G. et al. Label-free mid-infrared photothermal live-cell imaging beyond video rate. Light: Science & Applications, 12, 1661-1674 (2023). DOI： 10.1038/s41377-023-01214-2.

摘要

Abstract

Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy

such as liquid biopsy or breath diagnosis. On the contrary

MIR microscopy has been rarely used for live biological samples in an aqueous environment due to the lack of spatial resolution and the large water absorption background. Recently

mid-infrared photothermal (MIP) imaging has proven to be applicable to 2D and 3D single-cell imaging with high spatial resolution inherited from visible light. However

the maximum measurement rate has been limited to several frames s

−1

limiting its range of use. Here

we develop a significantly improved wide-field MIP quantitative phase microscope with two orders-of-magnitude higher signal-to-noise ratio than previous MIP imaging techniques and demonstrate live-cell imaging beyond video rate. We first derive optimal system design by numerically simulating thermal conduction following the photothermal effect. Then

we develop the designed system with a homemade nanosecond MIR optical parametric oscillator and a high full-well-capacity image sensor. Our high-speed and high-spatial-resolution MIR microscope has great potential to become a new tool for life science

in particular for live-cell analysis.

关键词

Keywords

references

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