Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography

Sungsam Kang; Renjie Zhou; Marten Brelen; Heather K. Mak; Yuechuan Lin; Peter T. C. So; Zahid Yaqoob

doi:10.1038/s41377-023-01240-0

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Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography

Original Articles

- Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography
- Light: Science & Applications Vol. 12, Issue 9, Pages: 1877-1885(2023)
- Affiliations：
  
  1.Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
  2.Department of Biomedical Engineering, The Chinese University of Hong Kong, Hong Kong, China
  3.Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, China
  4.Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
  5.Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
  6.Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA
- Author bio：
  
  Renjie Zhou (rjzhou@cuhk.edu.hk)
  Zahid Yaqoob (zyaqoob@mit.edu)
- Funds：
- DOI：10.1038/s41377-023-01240-0
  CLC：
- Published：30 September 2023，
  
  Published Online：22 August 2023，
  
  Received：23 March 2023，
  
  Revised：11 July 2023，
  
  Accepted：19 July 2023
- Accepted：
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Kang, S. et al. Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography. Light: Science & Applications, 12, 1877-1885 (2023).
DOI：

Kang, S. et al. Mapping nanoscale topographic features in thick tissues with speckle diffraction tomography. Light: Science & Applications, 12, 1877-1885 (2023). DOI： 10.1038/s41377-023-01240-0.

摘要

Abstract

Resolving three-dimensional morphological features in thick specimens remains a significant challenge for label-free imaging. We report a new speckle diffraction tomography (SDT) approach that can image thick biological specimens with ~500 nm lateral resolution and ~1 μm axial resolution in a reflection geometry. In SDT

multiple-scattering background is rejected through spatiotemporal gating provided by dynamic speckle-field interferometry

while depth-resolved refractive index maps are reconstructed by developing a comprehensive inverse-scattering model that also considers specimen-induced aberrations. Benefiting from the high-resolution and full-field quantitative imaging capabilities of SDT

we successfully imaged red blood cells and quantified their membrane fluctuations behind a turbid medium with a thickness of 2.8 scattering mean-free paths. Most importantly

we performed volumetric imaging of cornea inside an ex vivo rat eye and quantified its optical properties

including the mapping of nanoscale topographic features of Dua's and Descemet's membranes that had not been previously visualized.

关键词

Keywords

references

Popescu, G.Quantitative Phase Imaging of Cells and Tissues(McGraw Hill, 2011).

Park, Y., Depeursinge, C.&Popescu, G. Quantitative phase imaging in biomedicine.Nat. Photonics12, 578–589 (2018)..

Mir, M. et al. Optical measurement of cycle-dependent cell growth.Proc. Natl Acad. Sci. USA108, 13124–13129 (2011)..

Cooper, K. L. et al. Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions.Nature495, 375–378 (2013)..

Popescu, G. et al. Diffraction phase microscopy for quantifying cell structure and dynamics.Opt. Lett.31, 775–777 (2006)..

Popescu, G. et al. Imaging red blood cell dynamics by quantitative phase microscopy.Blood Cells Mol. Dis.41, 10–16 (2008)..

Park, Y. et al. Refractive index maps and membrane dynamics of human red blood cells parasitized byPlasmodium falciparum.Proc. Natl Acad. Sci. USA105, 13730–13735 (2008)..

Hosseini, P. et al. Cellular normoxic biophysical markers of hydroxyurea treatment in sickle cell disease.Proc. Natl Acad. Sci. USA113, 9527–9532 (2016)..

Fang-Yen, C. et al. Noncontact measurement of nerve displacement during action potential with a dual-beam low-coherence interferometer.Opt. Lett.29, 2028–2030 (2004)..

Oh, S. et al. Label-free imaging of membrane potential using membrane electromotility.Biophys. J.103, 11–18 (2012)..

Marquet, P., Depeursinge, C.&Magistretti, P. J. Review of quantitative phase-digital holographic microscopy: promising novel imaging technique to resolve neuronal network activity and identify cellular biomarkers of psychiatric disorders.Neurophotonics1, 020901 (2014)..

Subramanian, H. et al. Optical methodology for detecting histologically unapparent nanoscale consequences of genetic alterations in biological cells.Proc. Natl Acad. Sci. USA105, 20118–20123 (2008)..

Cherkezyan, L. et al. Interferometric spectroscopy of scattered light can quantify the statistics of subdiffractional refractive-index fluctuations.Phys. Rev. Lett.111, 033903 (2013)..

Wang, Z. et al. Tissue refractive index as marker of disease.J. Biomed. Opt.16, 116017 (2011)..

Liu, P. Y. et al. Cell refractive index for cell biology and disease diagnosis: past, present and future.Lab Chip16, 634–644 (2016)..

Charrière, F. et al. Cell refractive index tomography by digital holographic microscopy.Opt. Lett.31, 178–180 (2006)..

Choi, W. et al. Tomographic phase microscopy.Nat. Methods4, 717–719 (2007)..

Descloux, A. et al. Combined multi-plane phase retrieval and super-resolution optical fluctuation imaging for 4D cell microscopy.Nat. Photonics12, 165–172 (2018)..

Dong, D. S. et al. Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome.Light Sci. Appl.9, 11 (2020)..

Kwon, S. et al. Mitochondria-targeting indolizino [3, 2-c]quinolines as novel class of photosensitizers for photodynamic anticancer activity.Eur. J. Med. Chem.148, 116–127 (2018)..

Lee, M. et al. Deep-learning-based three-dimensional label-free tracking and analysis of immunological synapses of CAR-T cells.eLife9, e49023 (2020)..

Cotte, Y. et al. Marker-free phase nanoscopy.Nat. Photonics7, 113–117 (2013)..

Hosseini, P. et al. Scanning color optical tomography (SCOT).Opt. Express23, 19752–19762 (2015)..

Lue, N. et al. Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion.Opt. Express16, 16240–16246 (2008)..

Kim, T. et al. White-light diffraction tomography of unlabelled live cells.Nat. Photonics8, 256–263 (2014)..

Wolf, E. Three-dimensional structure determination of semi-transparent objects from holographic data.Opt. Commun.1, 153–156 (1969)..

Sung, Y. et al. Optical diffraction tomography for high resolution live cell imaging.Opt. Express17, 266–277 (2009)..

Sung, Y. et al. Stain-free quantification of chromosomes in live cells using regularized tomographic phase microscopy.PLoS ONE7, e49502 (2012)..

Weissleder, R. A clearer vision for in vivo imaging.Nat. Biotechnol.19, 316–317 (2001)..

Lim, J. et al. Comparative study of iterative reconstruction algorithms for missing cone problems in optical diffraction tomography.Opt. Express23, 16933–16948 (2015)..

Jin, D. et al. Tomographic phase microscopy: principles and applications in bioimaging [invited].J. Opt. Soc. Am. B34, B64–B77 (2017)..

Kamilov, U. S. et al. Learning approach to optical tomography.Optica2, 517–522 (2015)..

Tian, L.&Waller, L. 3D intensity and phase imaging from light field measurements in an LED array microscope.Optica2, 104–111 (2015)..

Lim, J. et al. High-fidelity optical diffraction tomography of multiple scattering samples.Light Sci. Appl.8, 82 (2019)..

Chen, M. et al. Multi-layer Born multiple-scattering model for 3D phase microscopy.Optica7, 394–403 (2020)..

Yaqoob, Z. et al. Single-shot full-field reflection phase microscopy.Opt. Express19, 7587–7595 (2011)..

Singh, V. R. et al. Studying nucleic envelope and plasma membrane mechanics of eukaryotic cells using confocal reflectance interferometric microscopy.Nat. Commun.10, 3652 (2019)..

Choi, Y. et al. Dynamic speckle illumination wide-field reflection phase microscopy.Opt. Lett.39, 6062–6065 (2014)..

Shemonski, N. D. et al. Computational high-resolution optical imaging of the living human retina.Nat. Photonics9, 440–443 (2015)..

Kandel, M. E. et al. Epi-illumination gradient light interference microscopy for imaging opaque structures.Nat. Commun.10, 4691 (2019)..

Kim, M. et al. Label-free neuroimaging in vivo using synchronous angular scanning microscopy with single-scattering accumulation algorithm.Nat. Commun.10, 3152 (2019)..

Ledwig, P.&Robles, F. E. Quantitative 3D refractive index tomography of opaque samples in epi-mode.Optica8, 6–14 (2021)..

Choi, Y. et al. Reflection phase microscopy using spatio-temporal coherence of light.Optica5, 1468–1473 (2018)..

Zhou, R. et al. Modeling the depth-sectioning effect in reflection-mode dynamic speckle-field interferometric microscopy.Opt. Express25, 130–143 (2017)..

Dua, H. S. et al. Human corneal anatomy redefined: a novel pre-Descemet's layer (Dua's layer).Ophthalmology120, 1778–1785 (2013)..

Eghrari, A. O., Riazuddin, S. A.&Gottsch, J. D. Overview of the cornea: structure, function, and development.Prog. Mol. Biol. Transl. Sci.134, 7–23 (2015)..

Booth, M. J., Neil, M. A. A.&Wilson, T. Aberration correction for confocal imaging in refractive‐index‐mismatched media.J. Microsc.192, 90–98 (1998)..

Son, M. et al. Effects of osmolality and solutes on the morphology of red blood cells according to three-dimensional refractive index tomography.PLoS ONE16, e0262106 (2021)..

Zhou, Y. F. et al. Characterizing refractive index and thickness of biological tissues using combined multiphoton microscopy and optical coherence tomography.Biomed. Opt. Express4, 38–50 (2013)..

Richardson, W. H. Bayesian-based iterative method of image restoration.J. Opt. Soc. Am.62, 55–59 (1972)..

Lucy, L. B. An iterative technique for the rectification of observed distributions.Astron. J.79, 745 (1974)..

Roberts, B. A.&Kak, A. C. Reflection mode diffraction tomography.Ultrason. Imaging7, 300–320 (1985)..

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