Label-free biosensing with singular-phase-enhanced lateral position shift based on atomically thin plasmonic nanomaterials

Shaodi Zhu; Rodolphe Jaffiol; Aurelian Crunteanu; Cyrille Vézy; Sik-To Chan; Wu Yuan; Ho-Pui Ho; Shuwen Zeng

doi:10.1038/s41377-023-01345-6

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Label-free biosensing with singular-phase-enhanced lateral position shift based on atomically thin plasmonic nanomaterials

Original Articles

- Label-free biosensing with singular-phase-enhanced lateral position shift based on atomically thin plasmonic nanomaterials
- Light: Science & Applications Vol. 13, Issue 5, Pages: 810-822(2024)
- Affiliations：
  
  1.Light, Nanomaterials & Nanotechnologies (L2n), CNRS-EMR 7004, University of Technology of Troyes, 10000 Troyes, France
  2.Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
  3.XLIM Research Institute, UMR 7252 CNRS/University of Limoges, 123, Avenue Albert Thomas, Limoges, France
- Author bio：
  
  Ho-Pui Ho (aaron.ho@cuhk.edu.hk)
  Shuwen Zeng (shuwen.zeng@cnrs.fr)
- Funds：
- DOI：10.1038/s41377-023-01345-6
  CLC：
- Received：02 July 2023，
  
  Revised：2023-11-18，
  
  Accepted：24 November 2023，
  
  Published Online：01 January 2024，
  
  Published：31 May 2024
- Accepted：
Scan QR Code
Zhu, S. D. et al. Label-free biosensing with singular-phase-enhanced lateral position shift based on atomically thin plasmonic nanomaterials. Light: Science & Applications, 13, 810-822 (2024).
DOI：

Zhu, S. D. et al. Label-free biosensing with singular-phase-enhanced lateral position shift based on atomically thin plasmonic nanomaterials. Light: Science & Applications, 13, 810-822 (2024). DOI： 10.1038/s41377-023-01345-6.

摘要

Abstract

Rapid plasmonic biosensing has attracted wide attention in early disease diagnosis and molecular biology research. However

it was still challenging for conventional angle-interrogating plasmonic sensors to obtain higher sensitivity without secondary amplifying labels such as plasmonic nanoparticles. To address this issue

we developed a plasmonic biosensor based on the enhanced lateral position shift by phase singularity. Such singularity presents as a sudden phase retardation at the dark point of reflection from resonating plasmonic substrate

leading to a giant position shift on reflected beam. Herein

for the first time

the atomically thin layer of Ge

(GST) on silver nanofilm was demonstrated as a novel phase-response-enhancing plasmonic material. The GST layer was not only precisely engineered to singularize phase change but also served as a protective layer for active silver nanofilm. This new configuration has achieved a record-breaking largest position shift of 439.3 μm measured in calibration experiments with an ultra-high sensitivity of 1.72 × 10

nm RIU

−1

(refractive index unit). The detection limit was determined to be 6.97 × 10

−7

RIU with a 0.12 μm position resolution. Besides

a large figure of merit (FOM) of 4.54 × 10

μm (RIU∙°)

−1

was evaluated for such position shift interrogation

enabling the labelfree detection of trace amounts of biomolecules. In targeted biosensing experiments

the optimized sensor has successfully detected small cytokine biomarkers (TNF-α and IL-6) with the lowest concentration of 1 × 10

−16

M. These two molecules are the key proinflammatory cancer markers in clinical diagnosis

which cannot be directly screened by current clinical techniques. To further validate the selectivity of our sensing systems

we also measured the affinity of integrin binding to arginylglycylaspartic acid (RGD) peptide (a key protein interaction in cell adhesion) with different Mn

ion concentrations

ranging from 1 nM to 1 mM.

关键词

Keywords

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