Free-moving-state microscopic imaging of cerebral oxygenation and hemodynamics with a photoacoustic fiberscope

Xiaoxuan Zhong; Yizhi Liang; Xiaoyu Wang; Haoying Lan; Xue Bai; Long Jin; Bai-Ou Guan

doi:10.1038/s41377-023-01348-3

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Free-moving-state microscopic imaging of cerebral oxygenation and hemodynamics with a photoacoustic fiberscope

Article

- Free-moving-state microscopic imaging of cerebral oxygenation and hemodynamics with a photoacoustic fiberscope
- Light: Science & Applications Vol. 13, Issue 1, Pages: 33-46(2024)
- Affiliations：
- Author bio：
  
  ftjinlong@jnu.edu.cn
  gtguanbo@jnu.edu.cn
- Funds：
- DOI：10.1038/s41377-023-01348-3
  CLC：
- Received：26 June 2023，
  
  Revised：22 November 2023，
  
  Accepted：2023-11-29，
  
  Published Online：02 January 2024，
  
  Published：2024-12
- Accepted：
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Xiaoxuan Zhong, Yizhi Liang, Xiaoyu Wang, et al. Free-moving-state microscopic imaging of cerebral oxygenation and hemodynamics with a photoacoustic fiberscope[J]. Light: science & applications, 2024, 13(1): 33-46.
DOI：

Xiaoxuan Zhong, Yizhi Liang, Xiaoyu Wang, et al. Free-moving-state microscopic imaging of cerebral oxygenation and hemodynamics with a photoacoustic fiberscope[J]. Light: science & applications, 2024, 13(1): 33-46. DOI： 10.1038/s41377-023-01348-3.

摘要

Abstract

We report the development of a head-mounted photoacoustic fiberscope for cerebral imaging in a freely behaving mouse. The 4.5-gram imaging probe has a 9-µm lateral resolution and 0.2-Hz frame rate over a 1.2-mm wide area.

The probe can continuously monitor cerebral oxygenation and hemodynamic responses at single-vessel resolution

showing significantly different cerebrovascular responses to external stimuli under anesthesia and in the freely moving state. For example

when subjected to high-concentration CO

respiration

enhanced oxygenation to compensate for hypercapnia can be visualized due to cerebral regulation in the freely moving state. Comparative studies exhibit significantly weakened compensation capabilities in obese rodents. This new imaging modality can be used for investigating both normal and pathological cerebrovascular functions and shows great promise for studying cerebral activity

disorders and their treatments.

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references

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