Transient stimulated Raman scattering spectroscopy and imaging

Qiaozhi Yu; Zhengjian Yao; Jiaqi Zhou; Wenhao Yu; Chenjie Zhuang; Yafeng Qi; Hanqing Xiong

doi:10.1038/s41377-024-01412-6

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Transient stimulated Raman scattering spectroscopy and imaging

Original Articles

- Transient stimulated Raman scattering spectroscopy and imaging
- Light: Science & Applications Vol. 13, Issue 4, Pages: 654-663(2024)
- Affiliations：
  
  1.National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China
  2.Biomedical Engineering Department, College of Future Technology, Peking University, Beijing 100871, China
- Author bio：
  
  Hanqing Xiong (xiong.hanqing@pku.edu.cn)
- Funds：
- DOI：10.1038/s41377-024-01412-6
  CLC：
- Received：15 September 2023，
  
  Revised：05 February 2024，
  
  Accepted：2024-02-17，
  
  Published Online：08 March 2024，
  
  Published：30 April 2024
- Accepted：
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Yu, Q. Z. et al. Transient stimulated Raman scattering spectroscopy and imaging. Light: Science & Applications, 13, 654-663 (2024).
DOI：

Yu, Q. Z. et al. Transient stimulated Raman scattering spectroscopy and imaging. Light: Science & Applications, 13, 654-663 (2024). DOI： 10.1038/s41377-024-01412-6.

摘要

Abstract

Stimulated Raman scattering (SRS) has been developed as an essential quantitative contrast for chemical imaging in recent years. However

while spectral lines near the natural linewidth limit can be routinely achieved by state-of-the-art spontaneous Raman microscopes

spectral broadening is inevitable for current mainstream SRS imaging methods. This is because those SRS signals are all measured in the frequency domain. There is a compromise between sensitivity and spectral resolution: as the nonlinear process benefits from pulsed excitations

the fundamental time-energy uncertainty limits the spectral resolution. Besides

the spectral range and acquisition speed are mutually restricted. Here we report transient stimulated Raman scattering (T-SRS)

an alternative time-domain strategy that bypasses all these fundamental conjugations. T-SRS is achieved by quantum coherence manipulation: we encode the vibrational oscillations in the stimulated Raman loss (SRL) signal by femtosecond pulse-pair sequence excited vibrational wave packet interference. The Raman spectrum was then achieved by Fourier transform of the time-domain SRL signal. Since all Raman modes are impulsively and simultaneously excited

T-SRS features the natural-linewidth-limit spectral line shapes

laser-bandwidth-determined spectral range

and improved sensitivity. With ~150-fs laser pulses

we boost the sensitivity of typical Raman modes to the sub-mM level. With all-plane-mirror high-speed time-delay scanning

we further demonstrated hyperspectral SRS imaging of live-cell metabolism and high-density multiplexed imaging with the natural-linewidth-limit spectral resolution. T-SRS shall find valuable applications for advanced Raman imaging.

关键词

Keywords

references

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