Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation

Shulang Lin; Li Gong; Zhiwei Huang

doi:10.1038/s41377-024-01498-y

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Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation

Original Articles

- Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation
- Light: Science & Applications Vol. 13, Issue 7, Pages: 1427-1440(2024)
- Affiliations：
  
  Optical Bioimaging Laboratory, Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117576, Singapore
- Author bio：
  
  Zhiwei Huang (biehzw@nus.edu.sg)
- Funds：
- DOI：10.1038/s41377-024-01498-y
  CLC：
- Published：31 July 2024，
  
  Published Online：01 July 2024，
  
  Received：26 December 2023，
  
  Revised：20 May 2024，
  
  Accepted：30 May 2024
- Accepted：
Scan QR Code
Lin, S. L., Gong, L. & Huang, Z. W. Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation.,Light: Science & Applications, 13, 1427-1440 (2024).
DOI：

Lin, S. L., Gong, L. & Huang, Z. W. Time-of-flight resolved stimulated Raman scattering microscopy using counter-propagating ultraslow Bessel light bullets generation.,Light: Science & Applications, 13, 1427-1440 (2024). DOI： 10.1038/s41377-024-01498-y.

摘要

Abstract

We present a novel time-of-flight resolved Bessel light bullet-enabled stimulated Raman scattering (B

-SRS) microscopy for deeper tissue 3D chemical imaging with high resolution without a need for mechanical z-scanning. To accomplish the tasks

we conceive a unique method to enable optical sectioning by generating t

he counter-propagating pump and Stokes Bessel light bullets in the sample

in which the group velocities of the Bessel light bullets are made ultraslow (e.g.

≈ 0.1c) and tunable by introducing programmable angular dispersions with a spatial light modulator. We theoretically analyze the working principle of the collinear multicolor Bessel light bullet generations and velocity controls with the relative time-of-flight resolved detection for SRS 3D deep tissue imaging. We have also built the B

-SRS imaging system and present the first demonstration of B

-SRS microscopy with Bessel light bullets for 3D chemical imaging in a variety of samples (e.g.

polymer bead phantoms

biological samples such as spring onion tissue and porcine brain) with high resolution. The B

-SRS technique provides a

2-fold improvement in imaging depth in porcine brain tissue compared to conventional SRS microscopy. The method of optical sectioning in tissue using counter-propagating ultraslow Bessel light bullets developed in B

-SRS is generic and easy to perform and can be readily extended to other nonlinear optical imaging modalities to advance 3D microscopic imaging in biological and biomedical systems and beyond.

关键词

Keywords

references

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