Visualizing mitochondrial dynamics at the nanoscale

Till Stephan; Peter Ilgen; Stefan Jakobs

doi:10.1038/s41377-024-01582-3

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Visualizing mitochondrial dynamics at the nanoscale

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- Visualizing mitochondrial dynamics at the nanoscale
- Light: Science & Applications Vol. 13, Issue 11, Pages: 2421-2423(2024)
- Affiliations：
  
  1.Buchmann Institute for Molecular Life Sciences, Goethe University Frankfurt am Main, Frankfurt am Main 60438, Germany
  2.Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, 37077 Göttingen, Germany
  3.Clinic of Neurology, University Medical Center Göttingen, Göttingen 37075, Germany
  4.Fraunhofer Institute for Translational Medicine and Pharmacology, Translational Neuroinflammation and Automated Microscopy, Göttingen 37075, Germany
- Author bio：
  
  Till Stephan (t.stephan@em.uni-frankfurt.de)
  Stefan Jakobs (sjakobs@gwdg.de)
- Funds：
- DOI：10.1038/s41377-024-01582-3
  CLC：
- Published：30 November 2024，
  
  Published Online：09 September 2024，
- Accepted：
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Stephan, T., Ilgen, P. & Jakobs, S. Visualizing mitochondrial dynamics at the nanoscale. Light: Science & Applications, 13, 2421-2423 (2024).
DOI：

Stephan, T., Ilgen, P. & Jakobs, S. Visualizing mitochondrial dynamics at the nanoscale. Light: Science & Applications, 13, 2421-2423 (2024). DOI： 10.1038/s41377-024-01582-3.

摘要

Abstract

The study of mitochondria is a formidable challenge for super-resolution microscopy due to their dynamic nature and complex membrane architecture. In this issue

Ren et al. introduce HBmito Crimson

a fluorogenic and photostable mitochondrial probe for STED microscopy and investigate how mitochondrial dynamics influence the spatial organization of mitochondrial DNA.

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Keywords

references

Jakobs, S., Stephan, T., Ilgen, P.&Brüser, C. Light microscopy of mitochondria at the nanoscale.Annu. Rev. Biophys.49, 289–308 (2020)..

Spahn, C., Grimm, J. B., Lavis, L. D., Lampe, M.&Heilemann, M. Whole-cell, 3D, and multicolor STED imaging with exchangeable fluorophores.Nano Lett.19, 500–505 (2019)..

Stephan, T., Roesch, A., Riedel, D.&Jakobs, S. Live-cell STED nanoscopy of mitochondrial cristae.Sci. Rep.9, 12419 (2019)..

Liu, T. et al. Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.Proc. Natl Acad. Sci. USA119, e2215799119, https://doi.org/10.1073/pnas.2215799119 (2022)..

Sun, J. et al. Super-resolution imaging of mitochondrial cristae using a more hydrophobic far-red Si-rhodamine probe.Chem. Commun.59, 13038–13041 (2023)..

Wang, C. et al. A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae.Proc. Natl Acad. Sci. USA116, 15817–15822 (2019)..

Yang, X. et al. Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe.Nat. Commun.11, 3699 (2020)..

Zheng, S. et al. Long-term super-resolution inner mitochondrial membrane imaging with a lipid probe.Nat. Chem. Biol.20, 83–92 (2024)..

Ren, W. et al. Visualization of cristae and mtDNA interactions via STED nanoscopy using a low saturation power probe.Light Sci. Appl.13, 116 (2024)..

Bonekamp, N. A.&Larsson, N. -G. SnapShot: mitochondrial nucleoid.Cell172, 388–388 (2018)..

Huang, X. et al. Fast, long-term, super-resolution imaging with Hessian structured illumination microscopy.Nat. Biotechnol.36, 451–459 (2018)..

Kondadi, A. K., Anand, R.&Reichert, A. S. Cristae membrane dynamics—a paradigm change.Trends Cell Biol.30, 923–936 (2020)..

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