Quantitative phase microscopies: accuracy comparison

Patrick C. Chaumet; Pierre Bon; Guillaume Maire; Anne Sentenac; Guillaume Baffou

doi:10.1038/s41377-024-01619-7

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Quantitative phase microscopies: accuracy comparison

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- Quantitative phase microscopies: accuracy comparison
- Light: Science & Applications Vol. 13, Issue 12, Pages: 2912-2940(2024)
- Affiliations：
  
  1.Institut Fresnel, CNRS, Aix Marseille Univ, Centrale Med, Marseille, France
  2.Université de Limoges, CNRS, XLIM, UMR 7252, F-87000 Limoges, France
  3.Neurotechnology Center, Department of Biological Sciences, Columbia University, New York, NY 10027, USA
- Author bio：
  
  Guillaume Baffou (guillaume.baffou@fresnel.fr)
- Funds：
- DOI：10.1038/s41377-024-01619-7
  CLC：
- Received：01 December 2023，
  
  Revised：02 August 2024，
  
  Accepted：2024-09-01，
  
  Published Online：11 October 2024，
  
  Published：31 December 2024
- Accepted：
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Chaumet, P. C. et al. Quantitative phase microscopies: accuracy comparison. Light: Science & Applications, 13, 2912-2940 (2024).
DOI：

Chaumet, P. C. et al. Quantitative phase microscopies: accuracy comparison. Light: Science & Applications, 13, 2912-2940 (2024). DOI： 10.1038/s41377-024-01619-7.

摘要

Abstract

Quantitative phase microscopies (QPMs) play a pivotal role in bio-imaging

offering unique insights that complement fluorescence imaging. They provide essential data on mass distribution and transport

inaccessible to fluorescence techniques. Additionally

QPMs are label-free

eliminating concerns of photobleaching and phototoxicity. However

navigating through the array of available QPM techniques can be complex

making it challenging to select the most suitable one for a particular application. This tutorial review presents a thorough comparison of the main QPM techniques

focusing on their accuracy in terms of measurement precision and trueness. We focus on 8 techniques

namely digital holographic microscopy (DHM)

cross-grating wavefront microscopy (CGM)

which is based on QLSI (quadriwave lateral shearing interferometry)

diffraction phase microscopy (DPM)

differential phase-contrast (DPC) microscopy

phase-shifting interferometry (PSI) imaging

Fourier phase microscopy (FPM)

spatial light interference microscopy (SLIM)

and transport-of-intensity equation (TIE) imaging. For this purpose

we used a home-made numerical toolbox based on discrete dipole approximation (IF-DDA). This toolbox is designed to compute the electromagnetic field at the sample plane of a microscope

irrespective of the object's complexity or the illumination conditions. We upgraded this toolbox to enable it to model any type of QPM

and to take into account shot noise. In a nutshell

the results show that DHM and PSI are inherently free from artefacts and rather suffer from coherent noise; In CGM

DPC

DPM and TIE

there is a trade-off between precision and trueness

which can be balanced by varying one experimental parameter; FPM and SLIM suffer from inherent artefacts that cannot be discarded experimentally in most cases

making the techniques not quantitative especially for large objects covering a large part of the field of view

such as eukaryotic cells.

关键词

Keywords

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