Optical sectioning methods in three-dimensional bioimaging

Jing Zhang; Wei Qiao; Rui Jin; Hongjin Li; Hui Gong; Shih-Chi Chen; Qingming Luo; Jing Yuan

doi:10.1038/s41377-024-01677-x

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Optical sectioning methods in three-dimensional bioimaging

Review

- Optical sectioning methods in three-dimensional bioimaging
- Light: Science & Applications Vol. 14, Issue 1, Pages: 47-74(2025)
- Affiliations：
  
  1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China
  2.MoE Key Laboratory for Biomedical Photonics, Innovation Institute, Huazhong University of Science and Technology, Wuhan, China
  3.Hong Kong Center for Cerebro-Cardiovascular Health Engineering, N.T, Hong Kong, China
  4.Department of Biomedical Engineering, City University of Hong Kong, Hong Kong, China
  5.HUST-Suzhou Institute for Brainsmatics, Suzhou, China
  6.Department of Mechanical and Automation Engineering, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
  7.School of Biomedical Engineering, Hainan University, Haikou, China
- Author bio：
  
  Shih-Chi Chen (scchen@mae.cuhk.edu.hk)
  Qingming Luo (qluo@hainanu.edu.cn)
  Jing Yuan (yuanj@hust.edu.cn)
- Funds：
- DOI：10.1038/s41377-024-01677-x
  CLC：
- Published：31 January 2025，
  
  Published Online：01 January 2025，
  
  Received：11 May 2024，
  
  Revised：24 September 2024，
  
  Accepted：2024-10-28
- Accepted：
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Zhang, J. et al. Optical sectioning methods in three-dimensional bioimaging. Light: Science & Applications, 14, 47-74 (2025).
DOI：

Zhang, J. et al. Optical sectioning methods in three-dimensional bioimaging. Light: Science & Applications, 14, 47-74 (2025). DOI： 10.1038/s41377-024-01677-x.

摘要

Abstract

In recent advancements in life sciences

optical microscopy has played a crucial role in acquiring high-quality three-dimensional structural and functional information. However

the quality of 3D images is often compromised due to the intense scattering effect in biological tissues

compounded by several issues such as limited spatiotemporal resolution

low signal-to-noise ratio

inadequate depth of penetration

and high phototoxicity. Although various optical sectioning techniques have been developed to address these challenges

each method adheres to distinct imaging principles for specific applications. As a result

the effective selection of suitable optical sectioning techniques across diverse imaging scenarios has become crucial yet challenging. This paper comprehensively overviews existing optical sectioning techniques and selection guidance under different imaging scenarios. Specifically

we categorize the microscope design based on the spatial relationship between the illumination and detection axis

i.e.

on-axis and off-axis. This classification provides a unique perspective to compare the implementation and performances of various optical sectioning approaches. Lastly

we integrate selected optical sectioning methods on a custom-built off-axis imaging system and present a unique perspective for the future development of optical sectioning techniques.

关键词

Keywords

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