MINFLUX nanoscopy enhanced with high-order vortex beams

Xiao-Jie Tan; Zhiwei Huang

doi:10.1038/s41377-025-01822-0

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MINFLUX nanoscopy enhanced with high-order vortex beams

Original Articles

- MINFLUX nanoscopy enhanced with high-order vortex beams
- Light: Science & Applications Vol. 14, Issue 7, Pages: 1893-1900(2025)
- Affiliations：
  
  1.Optical Bioimaging Laboratory, Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117576, Singapore
  2.National University of Singapore (Suzhou) Research Institute, Suzhou, Jiangsu 215123, China
  3.NUS Graduate School for Integrative Sciences and Engineering Programme (ISEP), National University of Singapore, Singapore 119077, Singapore
- Author bio：
  
  Zhiwei Huang (biehzw@nus.edu.sg)
- Funds：
- DOI：10.1038/s41377-025-01822-0
  CLC：
- Received：18 September 2024，
  
  Revised：20 February 2025，
  
  Accepted：09 March 2025，
  
  Published Online：06 May 2025，
  
  Published：31 July 2025
- Accepted：
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Tan, X. J. & Huang, Z. W. MINFLUX nanoscopy enhanced with high-order vortex beams. Light: Science & Applications, 14, 1893-1900 (2025).
DOI：

Tan, X. J. & Huang, Z. W. MINFLUX nanoscopy enhanced with high-order vortex beams. Light: Science & Applications, 14, 1893-1900 (2025). DOI： 10.1038/s41377-025-01822-0.

摘要

Abstract

Minimal photon fluxes (MINFLUX) nanoscopy has emerged as a transformative advancement in superresolution imaging

enabling unprecedented nanoscale observations across diverse biological scenarios. In this work

we propose

for the first time

that employing high-order vortex beams can significantly enhance the performance of MINFLUX

surpassing the limitations of the conventional MINFLUX using the first-order vortex beam. Our theoretical analysis indicates that

for standard MINFLUX

high-order vortex beams can improve the maximum localization precision by a factor corresponding to their order

which can approach a sub-nanometer scale under optimal conditions

and for raster scan MINFLUX

high-order vortex beams allow for a wider field of view while maintaining enhanced precision. These findings underscore the potential of high-order vortex beams to elevate the performance of MINFLUX

paving the way towards ultra-high resolution imaging for a broad range of applications.

关键词

Keywords

references

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