Toward noninvasive optoacoustic imaging of whole-heart dynamics in mice

Sandeep Kumar Kalva; Cagla Özsoy; Daniil Nozdriukhin; Savannah Tiemann; Lin Tang; Xosé Luís Deán-Ben; Daniel Razansky

doi:10.1038/s41377-025-01992-x

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Toward noninvasive optoacoustic imaging of whole-heart dynamics in mice

Original Articles

- Toward noninvasive optoacoustic imaging of whole-heart dynamics in mice
- Light: Science & Applications Vol. 14, Issue 12, Pages: 4145-4153(2025)
- Affiliations：
  
  1.Institute of Pharmacology and Toxicology and Institute for Biomedical Engineering, Faculty of Medicine, University of Zurich, Zurich, Switzerland
  2.Institute for Biomedical Engineering, Department of Information Technology and Electrical Engineering, ETH Zurich, Zurich, Switzerland
  3.Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Mumbai 400076, India
- Author bio：
  
  Daniel Razansky (daniel.razansky@uzh.ch)
- Funds：
- DOI：10.1038/s41377-025-01992-x
  CLC：
- Received：27 January 2025，
  
  Revised：2025-07-15，
  
  Accepted：25 July 2025，
  
  Published Online：27 November 2025，
  
  Published：31 December 2025
- Accepted：
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Kalva, S. K. et al. Toward noninvasive optoacoustic imaging of whole-heart dynamics in mice. Light: Science & Applications, 14, 4145-4153 (2025).
DOI：

Kalva, S. K. et al. Toward noninvasive optoacoustic imaging of whole-heart dynamics in mice. Light: Science & Applications, 14, 4145-4153 (2025). DOI： 10.1038/s41377-025-01992-x.

摘要

Abstract

High-speed volumetric optoacoustic tomography (VOT) offers powerful means for noninvasive

detailed visualization of rapid cardiac dynamics in mice. However

current implementations suffer from non-uniform light delivery into the thoracic area

which results in diminished penetration depth

limited field-of-view

and compromised quantification abilities. In this work

we devised a new VOT approach featuring hexagonally-shaped light delivery optimized for whole-heart imaging and an expedited imaging speed of 200 volumes per second using a custom-made spherical array transducer. The enhanced imaging performance was confirmed with calibration phantoms and noninvasive imaging of the murine heart. We capitalized on the reduced hemoglobin absorption in the second near-infrared (NIR-Ⅱ) spectral window to mitigate the strong light attenuation by whole blood within the cardiac chambers while further employing copper sulfide nanoparticles featuring a strong NIR-Ⅱ absorption to quantify cardiac functional parameters across the entire heart in vivo. The new approach can thus facilitate the monitoring of cardiac abnormalities and assessment of therapeutic interventions.

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references

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