Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view

Van Tu Nguyen; Carlos Taboada; Jesse Delia; Tri Vu; Luca Menozzi; Soon-Woo Cho; Jing Li; Nishad Jayasundara; Anthony DiSpirito; Junjie Yao

doi:10.1038/s41377-025-02114-3

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Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view

Original Articles

- Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view
- Light: Science & Applications Vol. 15, Issue 3, Pages: 996-1006(2026)
- Affiliations：
  
  1.Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA
  2.Biology Department, Duke University, Durham, NC 27708, USA
  3.Department of Biological Sciences, Vanderbilt University, Nashville, TN 37232, USA
  4.Nicholas School of the Environment, Duke University, Durham, NC 27713, USA
- Author bio：
  
  Carlos Taboada (carlos.a.taboada@vanderbilt.edu)
  Junjie Yao (junjie.yao@duke.edu)
- Funds：
- DOI：10.1038/s41377-025-02114-3
  CLC：
- Received：20 April 2025，
  
  Revised：2025-09-30，
  
  Accepted：31 October 2025，
  
  Online First：28 January 2026，
  
  Published：31 March 2026
- Accepted：
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Nguyen, V. T. et al. Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view. Light: Science & Applications, 15, 996-1006 (2026).
DOI：

Nguyen, V. T. et al. Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view. Light: Science & Applications, 15, 996-1006 (2026). DOI： 10.1038/s41377-025-02114-3.

摘要

Abstract

Photoacoustic microscopy (PAM) systems often face challenges in simultaneously achieving high speed

high resolution

high sensitivity

and a large field of view (FOV). To address this challenge

we have developed dual-channel PAM (DC-PAM) that can expand the FOV without compromising the imaging speed

detection sensitivity

or spatial resolution. DC-PAM has two identical

independent channels of laser excitation and acoustic detection. It exploits two facets of a single hexagon scanner to concurrently steer the dual excitation laser beams and the resultant acoustic waves. DC-PAM achieves an ultra-wide FOV of 22.5 × 24 mm

with a total functional imaging time of ~15 s. Proof-of-concept experiments were conducted using DC-PAM on freely-swimming zebrafish

hypoxia-challenged mice

and sleeping glassfrogs

all of which benefit from the large FOV and high imaging speed to track the dynamic and physiological processes at the whole-organ or whole-body level. These applications demonstrate the potential of DC-PAM for a wide range of biological studies.

关键词

Keywords

references

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