Holmes, D. F. et al. Corneal collagen fibril structure in three dimensions: structural insights into fibril assembly, mechanical properties, and tissue organization. Proc. Natl. Acad. Sci. USA 98 , 7307–7312 (200 1)..

Benjamin, M., Kaiser, E. & Milz, S. Structure-function relationships in tendons: a review. J. Anat. 212 , 211–228 (2008)..

Zeisberg, M. & Kalluri, R. Cellular mechanisms of tissue fibrosis. 1. Common and organ-specific mechanisms associated with tissue fibrosis. Am. J. Physiol.-Cell Physiol. 304 , C216–C225 (2013)..

Lichtman, J. W. & Conchello, J. A. Fluorescence microscopy. Nat. Methods 2 , 910–919 (2005)..

Parfitt, G. J. Immunofluorescence tomography: high-resolution 3-D reconstruction by serial-sectioning of methacrylate embedded tissues and alignment of 2-D immunofluorescence images. Sci. Rep. 9 , 1992 (2019)..

Lo, R. C. & Kim, H. Histopathological evaluation of liver fibrosis and cirrhosis regression. Clin. Mol. Hepatol. 23 , 302–307 (2017)..

Gong, Y. H., Chen, X. R. & Wu, W. Application of Fourier transform infrared (FTIR) spectroscopy in sample preparation: material characterization and mechanism investigation. Adv. Sample Preparation 11 , 100122 (2024)..

Aghigh, A. et al. Second harmonic generation microscopy: a powerful tool for bio-imaging. Biophysical Rev. 15 , 43–70 (2023)..

Zhang, W. X. et al. Multi-molecular hyperspectral PRM-SRS microscopy. Nat. Commun. 15 , 1599 (2024)..

Park, E. et al. Label-free mid-infrared dichroism-sensitive photoacoustic microscopy for histostructural analysis of engineered heart tissues. Light Sci. Appl. 15 , 49 (2026)..

Xu, M. H. & Wang, L. V. Photoacoustic imaging in biomedicine. Rev. Sci. Instrum. 77 , 041101 (2006)..

Kruger, R. A. et al. Photoacoustic ultrasound (PAUS)—reconstruction tomography. Med. Phys. 22 , 1605–1609 (1995)..

Gu, Y. R. et al. Application of photoacoustic computed tomography in biomedical imaging: a literature review. Bioeng. Transl. Med. 8 , e10419 (2023)..

Zhu, X. Y. et al. Real-time whole-brain imaging of hemodynamics and oxygenation at micro-vessel resolution with ultrafast wide-field photoacoustic microscopy. Light Sci. Appl. 11 , 138 (2022)..

Yao, J. J. et al. High-speed label-free functional photoacoustic microscopy of mouse brain in action. Nat. Methods 12 , 407–410 (2015)..

Wang, L. V. & Yao, J. J. A practical guide to photoacoustic tomography in the life sciences. Nat. Methods 13 , 627–638 (2016)..

Yao, J. J. et al. Label-free oxygen-metabolic photoacoustic microscopy in vivo. J. Biomed. Opt. 16 , 076003 (2011)..

He, Y. et al. Label-free imaging of lipid-rich biological tissues by mid-infrared photoacoustic microscopy. J. Biomed. Opt. 25 , 106506 (2020)..

Zhang, C. et al. Label-free photoacoustic microscopy of cytochromes. J. Biomed. Opt. 18 , 020504 (2013)..

Li, X. F. et al. Ultraviolet photoacoustic microscopy with tissue clearing for high-contrast histological imaging. Photoacoustics 25 , 100313 (2022)..

Kim, D. et al. An ultraviolet-transpa rent ultrasound transducer enables high-resolution label-free photoacoustic histopathology. Laser Photonics Rev. 18 , 2300652 (2024)..

Rong, Q. Z. et al. From ultravioletto near-infrared: label-free reflection-mode hyperspectral photoacoustic microscopy for single-cell biochemical mapping. GEN Biotechnol. 2 , 435–444 (2023)..

Rong, Q. Z. et al. Label-free photoacoustic imaging of glassfrog development. Photoacoustics 46 , 100773 (2025)..

Pleitez, M. A. et al. Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells. Nat. Biotechnol. 38 , 293–296 (2020)..

Visscher, M. et al. Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy. Photoacoustics 26 , 100354 (2022)..

Shi, J. H. et al. High-resolution, high-contrast mid-infrared imaging of fresh biological samples with ultraviolet-localized photoacoustic microscopy. Nat. Photonics 13 , 609–615 (2019)..

Zhang, D. L. et al. Depth-resolved mid-infrared photothermal imaging of living cells and organism s with submicrometer spatial resolution. Sci. Adv. 2 , e1600521 (2016)..

Yuan, T. et al. Functional live-cell mid-infrared microscopy and spectroscopy by optoacoustic and optothermal detection. Proceedings of SPIE 12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023. San Francisco, California, United States: SPIE, 2023, 1239202.

Qu, Y. et al. Dichroism-sensitive photoacoustic computed tomography. Optica 5 , 495–501 (2018)..

Zhou, Y. Y. et al. Single-shot linear dichroism optical-resolution photoacoustic microscopy. Photoacoustics 16 , 100148 (2019)..

Park, E. et al. Azimuth mapping of fibrous tissue in linear dichroism-sensitive photoacoustic microscopy. Photoacoustics 31 , 100510 (2023)..

Yoon, C. et al. Deep learning-based virtual staining, segmentation, and classification in label-free photoacoustic histology of human specimens. Light Sci. Appl. 13 , 226 (2024)..

De Boer, J. F., Hitzenberger, C. K. & Yasuno, Y. Polarization sensitive optical coherence tomography - a review [invited ] . Biomed. Opt. Express 8 , 1838 (2017)..

Wang, N. C. & Yao, J. J. Sound out the deep clarity: super-resolution photoacoustic imaging at depths. IEEE Trans. Ultrason., Ferroelectr., Frequency Control 71 , 1801–1813 (2024)..

Li, M. C. et al. Sound out the deep colors: photoacoustic molecular imaging at new depths. Mol. Imaging 19 , 1536012120981518 (2020)..