Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level

Zhen Ma; Qiuying Liu; Huaxiao Yang; Raymond B Runyan; Carol A Eisenberg; Meifeng Xu; Thomas K Borg; Roger Markwald; Yifei Wang; Bruce Z Gao

doi:10.1038/lsa.2013.24

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Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level

Original Articles

- Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level
- Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level
- LSA 2013年2卷第1-2期
- Affiliations：
  
  1.Department of Bioengineering and COMSET, Clemson University, Clemson, SC 29634, USA
  2.Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA
  3.Biomedical R&D Center, Jinan University, Guangzhou, China
  4.Department of Cellular and Molecular Medicine, University of Arizona, Tucson, AZ 85724, USA
  5.New York Medical College/Westchester Medical Center Stem Cell Laboratory, New York Medical College, Valhalla, New York, USA
  6.Department of Pathology and Laboratory Medicine, University of Cincinnati Medical Center, Cincinnati, OH, USA
  7.Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, USA
- Author bio：
  
  Professor BZ Gao, Department of Bioengineering and COMSET, Clemson University, 201-3 Rhodes Hall, Clemson, SC 29634, USA Email: zgao@clemson.edu
- Funds：
- DOI：10.1038/lsa.2013.24
  中图分类号：
- 纸质出版日期：2013-06，
  
  网络出版日期：2013-05-24，
  
  收稿日期：2012-09-22，
  
  修回日期：2013-01-03，
  
  录用日期：2013-01-07
- Accepted：
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Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level[J]. LSA, 2013,2(1-2).

Ma, Z. et al. Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level. Light: Science & Applications, 2, e68 (2013).
Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level[J]. LSA, 2013,2(1-2). DOI： 10.1038/lsa.2013.24.

Ma, Z. et al. Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level. Light: Science & Applications, 2, e68 (2013). DOI： 10.1038/lsa.2013.24.

摘要

Abstract

Mesenchymal stem cells (MSCs) have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions

including the paracrine effect

cell fusion

and mechanical and electrical couplings. Due to heart–muscle complexity

progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture. In conventional coculture systems

however

the

in vivo

cardiac muscle structure

in which rod-shaped cells are connected end-to-end

is not sustained; instead

irregularly shaped cells spread randomly

resulting in randomly distributed cell junctions. Consequently

contact-mediated cell–cell interactions (e.g.

the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions) occur randomly. Thus

the data generated on the beneficial effects of MSCs may be irrelevant to

in vivo

biological processes. In this study

we explored whether cardiomyocyte alignment

the most important phenotype

is relevant to stem cell cardiogenic differentiation. Here

we report (ⅰ) the construction of a laser-patterned

biochip-based

stem cell–cardiomyocyte coculture model with controlled cell alignment; and (ⅱ) single-cell-level data on stem cell cardiogenic differentiation under

in vivo

-like cardiomyocyte alignment conditions.

关键词

Keywords

cardiogenic differentiationmesenchymal stem cellsmicroenvironmentoptical forcesingle-cell analysis

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