Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy

Doekele G Stavenga; Hein L Leertouwer; Bodo D Wilts

doi:10.1038/lsa.2013.56

Go to Nature Website

Your Location：

Home >

Browse articles >

Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy

Original Articles

- Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy
- Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy
- LSA 2013年2卷第3-4期
- Affiliations：
  
  Computational Physics, Zernike Institute for Advanced Materials, University of Groningen, NL-9747 AG Groningen, The Netherlands
- Author bio：
  
  Professor DG Stavenga, Computational Physics, Zernike Institute for Advanced Materials, University of Groningen, NL-9747 AG Groningen, The Netherlands E-mail: D.G.Stavenga@rug.nl
- Funds：
- DOI：10.1038/lsa.2013.56
  中图分类号：
- 纸质出版日期：2013-12，
  
  网络出版日期：2013-09-27，
  
  收稿日期：2013-03-25，
  
  修回日期：2013-05-28，
  
  录用日期：2013-05-30
- Accepted：
Scan QR Code
Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy[J]. LSA, 2013,2(3-4).

Stavenga, D. G., Leertouwer, H. L. & Wilts, B. D. Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy. Light: Science & Applications, 2, e100 (2013).
Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy[J]. LSA, 2013,2(3-4). DOI： 10.1038/lsa.2013.56.

Stavenga, D. G., Leertouwer, H. L. & Wilts, B. D. Quantifying the refractive index dispersion of a pigmented biological tissue using Jamin–Lebedeff interference microscopy. Light: Science & Applications, 2, e100 (2013). DOI： 10.1038/lsa.2013.56.

摘要

Abstract

Jamin–Lebedeff polarizing interference microscopy is a classical method for determining the refractive index and thickness of transparent tissues. Here

we extend the application of this method to pigmented

absorbing biological tissues

based on a theoretical derivation using Jones calculus. This novel method is applied to the wings of the American Rubyspot damselfly

Hetaerina americana

. The membranes in the red-colored parts of the damselfly's wings

with a thickness of ~2.5 µm

contain a pigment with maximal absorption at ~490 nm and a peak absorbance coefficient of ~0.7 µm

−1

. The high pigment density causes a considerable and anomalous dispersion of the refractive index. This result can be quantitatively understood from the pigment absorbance spectrum by applying the Kramers–Kronig dispersion relations. Measurements of the spectral dependence of the refractive index and the absorption are valuable for gaining quantitative insight into how the material properties of animal tissues influence coloration.

关键词

Keywords

absorptionchitininsect wingsJones calculusKramers–Kronig relations

references

Saks L, McGraw KJ, Hõrak P. How feather colour reflects its carotenoid content.Funct Ecol2003;17: 555–561..

Shawkey MD, Hill GE. Carotenoids need structural colours to shine.Biol Lett2005;1: 121–124..

Wijnen B, Leertouwer HL, Stavenga DG. Colors and pterin pigmentation of pierid butterfly wings.J Insect Physiol2007;53: 1206–1217..

Vukusic P, Stavenga DG. Physical methods for investigating structural colours in biological systems.J R Soc Interface2009;6(Suppl 2): S133–S148..

Gillis JM, Wibo M. Accurate measurement of the thickness of ultrathin sections by interference microscopy.J Cell Biol1971;49: 947–949..

Schäfer FJ, Kleemann W. High precision refractive index measurements revealing order parameter fluctuations in KMnF3 and NiO.J Appl Phys1985;57: 2606–2612..

Stavenga DG, Kruizinga R, Leertouwer HL. Dioptrics of the facet lenses of male blowfliesCalliphoraandChrysomia.J Comp Physiol A1990;166: 365–371..

Leertouwer HL, Wilts BD, Stavenga DG. Refractive index and dispersion of butterfly scale chitin and bird feather keratin measured by interference microscopy.Opt Express2011;19: 24061–24066..

Stavenga DG, Tinbergen J, Leertouwer HL, Wilts BD. Kingfisher feathers—colouration by pigments, spongy nanostructures and thin films.J Exp Biol2001;214: 3960–3967..

Stavenga DG, Smits RP, Hoenders BJ. Simple exponential functions describing the absorbance bands of visual pigment spectra.Vision Res1993;33: 1011–1017..

Schneider CA, Rasband WS, Eliceiri KW. NIH Image to ImageJ: 25 years of image analysis.Nat Methods2012;9: 671–675..

Goldstein D.Polarized Light. New York/Basel: Marcel Dekker; 2003.

Bell EE. Optical constants and their measurements. In: Genzel L, editor.Handbuch der Physik. Vol. XXV/2a. Berlin: Springer; 1967. pp1–58.

Stavenga DG, van Barneveld HH. On dispersion in visual photoreceptors.Vision Res1975;15: 1091–1095..

Nijhout HF. Ommochrome pigmentation of the linea and rosa seasonal forms ofPrecis coenia(Lepidoptera: Nymphalidae).Arch Insect Biochem Physiol1997;36: 215–222..

Reed RD, Nagy LM. Evolutionary redeployment of a biosynthetic module: expression of eye pigment genes vermilion, cinnabar, and white in butterfly wing development.Evol Dev2005;7: 301–311..

Hooper IR, Vukusic P, Wootton RJ. Detailed optical study of the transparent wing membranes of the dragonflyAeshna cyanea.Opt Express2006;14: 4891–4897..

Exner S.Die Physiologie der facittirten Augen von Krebsen und Insecten. Leipzig: Deuticke; 1891.

Exner S.The Physiology of the Compound Eyes of Insects and Crustaceans(translated by R.C. Hardie). Berlin/Heidelberg: Springer; 1989.

Doughtie DG, Rao KR. Ultrastructure of the eyes of the grass shrimp,Palaemonetes pugio.Cell Tiss Res1984;238: 271–288..

Mäthger LM, Hanlon RT. Malleable skin coloration in cephalopods: selective reflectance, transmission and absorbance of light by chromatophores and iridophores.Cell Tiss Res2007;329: 179–186..

Stavenga DG, Leertouwer HL, Hariyama T, de Raedt HA, Wilts BD. Sexual dichromatism of the damselflyCalopteryx japonicacaused by a melanin-chitin multilayer in the male wing veins.PLoS ONE2012;7: e49743..

Durrer H. Schillerfarben der Vogelfeder als Evolutionsproblem.Denkschr Schweiz Naturforsch Ges1977;91: 1–126..

Stavenga DG, Leertouwer HL, Marshall NJ, Osorio D. Dramatic colour changes in a bird of paradise caused by uniquely structured breast feather barbules.Proc R Soc B2010;278: 2098–2104..

Kinoshita S, Yoshioka S, Miyazaki J. Physics of structural colors.Rep Prog Phys2008;71: 076401..

Hariyama T, Hironaka M, Takaku Y, Horiguchi H, Stavenga DG. The leaf beetle, the jewel beetle, and the damselfly; insects with a multilayered show case. In: KinoshitaS, Yoshioka S, editors.Structural Color in Biological Systems—Principles and Applications. Osaka: Osaka University Press; 2005. pp153–176..

Stavenga DG, Wilts BD, Leertouwer HL, Hariyama T. Polarized iridescence of the multilayered elytra of the Japanese Jewel Beetle,Chrysochroa fulgidissima.Phil Trans R Soc B2011;366: 709–723..

Land MF. The physics and biology of animal reflectors.Prog Biophys Mol Biol1972;24: 77–105..

Kinoshita S.Structural Colors in the Realm Of Nature. Singapore: World Scientific; 2008.

Wilts BD, Michielsen K, de Raedt H, Stavenga DG. Iridescence and spectral filtering of the gyroid-type photonic crystals inParides sesostriswing scales.Interface Focus2012;2: 681–687..

Trzeciak TM, Wilts BD, Stavenga DG, Vukusic P. Variable multilayer reflection together with long-pass filtering pigment determines the wing coloration of papilionid butterflies of thenireusgroup.Opt Express2012;20: 8877–8890..

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据