Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation

Ji Yi; Wenzhong Liu; Siyu Chen; Vadim Backman; Nader Sheibani; Christine M. Sorenson; Amani A. Fawzi; Robert A. Linsenmeier; Hao F. Zhang

doi:10.1038/lsa.2015.107

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Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation

Original Articles

- Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation
- Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation
- LSA 2015年4卷第4期
- Affiliations：
  
  1.Department of Biomedical Engineering, Northwestern University, Evanston, IL 60208, USA
  2.McPherson Eye Research Institute, University of Wisconsin, Madison, WI 53705, USA
  3.Department of Ophthalmology, Northwestern University, Chicago Illinois 60611, USA
  4.Department of Neurobiology, Northwestern University, Evanston, IL 60208, USA
- Author bio：
  
  HF Zhang, Email: hfzhang@northwestern.edu
- Funds：
- DOI：10.1038/lsa.2015.107
  中图分类号：
- 纸质出版日期：2015-12，
  
  网络出版日期：2015-06-10，
  
  收稿日期：2015-03-11，
  
  修回日期：2015-06-01，
  
  录用日期：2015-06-07
- Accepted：
Scan QR Code
Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation[J]. LSA, 2015,4(4).

Yi, J. et al. Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation. Light: Science & Applications, 4, e334 (2015).
Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation[J]. LSA, 2015,4(4). DOI： 10.1038/lsa.2015.107.

Yi, J. et al. Visible light optical coherence tomography measures retinal oxygen metabolic response to systemic oxygenation. Light: Science & Applications, 4, e334 (2015). DOI： 10.1038/lsa.2015.107.

摘要

Abstract

The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-related macular degeneration

diabetic retinopathy

and glaucoma. Using visible light optical coherence tomography (vis-OCT)

we demonstrated accurate and robust measurement of retinal oxygen metabolic rate (rMRO

) noninvasively in rat eyes. We continuously monitored the regulatory response of oxygen consumption to a progressive hypoxic challenge. We found that both oxygen delivery

and rMRO

increased from the highly regulated retinal circulation (RC) under hypoxia

by 0.28 ± 0.08 µL min

−1

(

0.001)

and 0.20 ± 0.04 µL min

−1

(

0.001) per 100 mmHg

systemic pO

reduction

respectively. The increased oxygen extraction compensated for the deficient oxygen supply from the poorly regulated choroidal circulation. Results from an oxygen diffusion model based on previous oxygen electrode measurements corroborated our

in vivo

observations. We believe that vis-OCT has the potential to reveal the fundamental role of oxygen metabolism in various retinal diseases.

关键词

Keywords

oxygen metabolismretinal circulationvisible light optical coherence tomography

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