Synthetic aperture-based on-chip microscopy

Wei Luo; Alon Greenbaum; Yibo Zhang; Aydogan Ozcan

doi:10.1038/lsa.2015.34

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Synthetic aperture-based on-chip microscopy

Original Articles

- Synthetic aperture-based on-chip microscopy
- Synthetic aperture-based on-chip microscopy
- LSA 2015年4卷第2期
- Affiliations：
  
  1.Electrical Engineering Department, University of California, Los Angeles, CA 90095, USA
  2.Bioengineering Department, University of California, Los Angeles, CA 90095, USA
  3.California NanoSystems Institute (CNSI), University of California, Los Angeles, CA 90095, USA
  4.Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
- Author bio：
  
  A Ozcan, 420 Westwood Plaza, Engr. IV 68-119, UCLA, Los Angeles, CA 90095, USA E-mail: ozcan@ucla.edu
- Funds：
- DOI：10.1038/lsa.2015.34
  中图分类号：
- 纸质出版日期：2015-06，
  
  网络出版日期：2014-12-23，
  
  收稿日期：2014-08-09，
  
  修回日期：2014-12-20，
  
  录用日期：2014-12-22
- Accepted：
Scan QR Code
Synthetic aperture-based on-chip microscopy[J]. LSA, 2015,4(2).

Luo, W. et al. Synthetic aperture-based on-chip microscopy. Light: Science & Applications, 4, e261 (2015).
Synthetic aperture-based on-chip microscopy[J]. LSA, 2015,4(2). DOI： 10.1038/lsa.2015.34.

Luo, W. et al. Synthetic aperture-based on-chip microscopy. Light: Science & Applications, 4, e261 (2015). DOI： 10.1038/lsa.2015.34.

摘要

Abstract

Wide field-of-view (FOV) and high-resolution imaging requires microscopy modalities to have large space-bandwidth products. Lensfree on-chip microscopy decouples resolution from FOV and can achieve a space-bandwidth product greater than one billion under unit magnification using state-of-the-art opto-electronic sensor chips and pixel super-resolution techniques. However

using vertical illumination

the effective numerical aperture (NA) that can be achieved with an on-chip microscope is limited by a poor signal-to-noise ratio (SNR) at high spatial frequencies and imaging artifacts that arise as a result of the relatively narrow acceptance angles of the sensor's pixels. Here

we report

for the first time

a synthetic aperture-based on-chip microscope in which the illumination angle is scanned across the surface of a dome to increase the effective NA of the reconstructed lensfree image to 1.4

achieving e.g.

~250-nm resolution at 700-nm wavelength under unit magnification. This synthetic aperture approach not only represents the largest NA achieved to date using an on-chip microscope but also enables color imaging of connected tissue samples

such as pathology slides

by achieving robust phase recovery without the need for multi-height scanning or any prior information about the sample. To validate the effectiveness of this

synthetic aperture-based

partially coherent

holographic on-chip microscope

we have successfully imaged color-stained cancer tissue slides as well as unstained Papanicolaou smears across a very large FOV of 20.5 mm

. This compact on-chip microscope based on a synthetic aperture approach could be useful for various applications in medicine

physical sciences and engineering that demand high-resolution wide-field imaging.

关键词

Keywords

computational imaginglensfree microscopyon-chip microscopysynthetic aperture

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