Ultrafast photonic PCR

Jun Ho Son; Byungrae Cho; SoonGweon Hong; Sang Hun Lee; Ori Hoxha; Amanda J Haack; Luke P Lee

doi:10.1038/lsa.2015.53

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Ultrafast photonic PCR

Original Articles

- Ultrafast photonic PCR
- Ultrafast photonic PCR
- LSA 2015年4卷第3期
- Affiliations：
  
  1.Department of Bioengineering, University of California, Berkeley, CA 94720, USA
  2.Berkeley Sensor and Actuator Center, University of California, Berkeley, CA 94720, USA
  3.Department of Electrical Engineering and Computer Sciences, University of California, Berkeley, CA 94720, USA
  4.Biophysics Graduate Program, University of California, Berkeley, CA 94720, USA
- Author bio：
  
  LP Lee, Email: lplee@berkeley.edu
- Funds：
- DOI：10.1038/lsa.2015.53
  中图分类号：
- 纸质出版日期：2015-09，
  
  网络出版日期：2015-06-29，
  
  收稿日期：2014-11-17，
  
  修回日期：2015-01-21，
  
  录用日期：2015-01-28
- Accepted：
Scan QR Code
Ultrafast photonic PCR[J]. LSA, 2015,4(3).

Son, J. H. et al. Ultrafast photonic PCR. Light: Science & Applications, 4, e280 (2015).
Ultrafast photonic PCR[J]. LSA, 2015,4(3). DOI： 10.1038/lsa.2015.53.

Son, J. H. et al. Ultrafast photonic PCR. Light: Science & Applications, 4, e280 (2015). DOI： 10.1038/lsa.2015.53.

摘要

Abstract

Nucleic acid amplification and quantification

via

polymerase chain reaction (PCR) is one of the most sensitive and powerful tools for clinical laboratories

precision medicine

personalized medicine

agricultural science

forensic science and environmental science. Ultrafast multiplex PCR

characterized by low power consumption

compact size and simple operation

is ideal for timely diagnosis at the point-of-care (POC). Although several fast/ultrafast PCR methods have been proposed

the use of a simple and robust PCR thermal cycler remains challenging for POC testing. Here

we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion

via

photon–electron–phonon coupling. We demonstrate an efficient photonic heat converter using a thin gold (Au) film due to its plasmon-assisted high optical absorption (approximately 65% at 450 nm

the peak wavelength of heat source light-emitting diodes (LEDs)). The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150 ℃ within 3 min. Using this method

ultrafast thermal cycling (30 cycles; heating and cooling rate of 12.79±0.93 ℃ s

−1

and 6.6±0.29 ℃ s

−1

respectively) from 55 ℃ (temperature of annealing) to 95 ℃ (temperature of denaturation) is accomplished within 5 min. Using photonic PCR thermal cycles

we demonstrate here successful nucleic acid (λ-DNA) amplification. Our simple

robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures

including on-chip thermal lysis and heating for isothermal amplifications.

关键词

Keywords

genomicslight-emitting diodes (LEDs)molecular diagnosticspersonalized medicineplasmonicspoint-of-care (POC) diagnosticspolymerase chain reaction (PCR)

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