Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators

Judith Su; Alexander FG Goldberg; Brian M Stoltz

doi:10.1038/lsa.2016.1

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Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators

Original Articles

- Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators
- Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators
- LSA 2016年5卷第1期
- Affiliations：
  
  1.Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA
  2.Divison of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA, USA
- Author bio：
  
  J Su, Email: judy@caltech.edu
- Funds：
- DOI：10.1038/lsa.2016.1
  中图分类号：
- 纸质出版日期：2016-03，
  
  网络出版日期：2015-08-22，
  
  收稿日期：2015-03-06，
  
  修回日期：2015-07-24，
  
  录用日期：2015-08-05
- Accepted：
Scan QR Code
Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators[J]. LSA, 2016,5(1).

Su, J., Goldberg, A. F. G. & Stoltz, B. M. Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators. Light: Science & Applications, 5, e16001 (2016).
Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators[J]. LSA, 2016,5(1). DOI： 10.1038/lsa.2016.1.

Su, J., Goldberg, A. F. G. & Stoltz, B. M. Label-free detection of single nanoparticles and biological molecules using microtoroid optical resonators. Light: Science & Applications, 5, e16001 (2016). DOI： 10.1038/lsa.2016.1.

摘要

Abstract

Single-molecule detection is one of the fundamental challenges of modern biology. Such experiments often use labels that can be expensive

difficult to produce

and for small analytes

might perturb the molecular events being studied. Analyte size plays an important role in determining detectability. Here we use laser-frequency locking in the context of sensing to improve the signal-to-noise ratio of microtoroid optical resonators to the extent that single nanoparticles 2.5 nm in radius

and 15.5 kDa molecules are detected in aqueous solution

thereby bringing these detectors to the size limits needed for detecting the key macromolecules of the cell. Our results

covering several orders of magnitude of particle radius (100 nm to 2 nm)

agree with the 'reactive' model prediction for the frequency shift of the resonator upon particle binding. This confirms that the main contribution of the frequency shift for the resonator upon particle binding is an increase in the effective path length due to part of the evanescent field coupling into the adsorbed particle. We anticipate that our results will enable many applications

including more sensitive medical diagnostics and fundamental studies of single receptor–ligand and protein–protein interactions in real time.

关键词

Keywords

biosensingfrequency lockinglabel-freemicrotoroidsingle molecule

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