Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy

Murad Omar; Johannes Rebling; Kai Wicker; Tobias Schmitt-Manderbach; Mathias Schwarz; Jérôme Gateau; Hérnan López-Schier; Timo Mappes; Vasilis Ntziachristos

doi:10.1038/lsa.2016.186

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Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy

Original Articles

- Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy
- Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy
- LSA 2017年6卷第1期
- Affiliations：
  
  1.Chair for Biological Imaging, Technische Universität München, München D-81675, Germany
  2.Institute for Biological and Medical Imaging, Helmholtz Zentrum München, Neuherberg D-85764, Germany
  3.Carl Zeiss AG, Corporate Research and Technology, Jena D-07745, Germany
  4.ESPCI Paris, PSL Research University, CNRS, INSERM, Institut Langevin, Paris F-75005, France
  5.Research Unit Sensory Biology and Organogenesis, Helmholtz Zentrum München, Neuherberg D-85764, Germany
  6.Carl Zeiss Vision International GmbH, Technology & Innovation, Aalen D-73430, Germany
- Author bio：
  
  M Omar, Email: murad.omar@tum.de
  V Ntziachristos, Email: v.ntziachristos@tum.de
- Funds：
- DOI：10.1038/lsa.2016.186
  中图分类号：
- 纸质出版日期：2017-03，
  
  网络出版日期：2016-07-11，
  
  收稿日期：2016-02-20，
  
  修回日期：2016-07-01，
  
  录用日期：2016-07-06
- Accepted：
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Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy[J]. LSA, 2017,6(1).

Omar, M. et al. Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy. Light: Science & Applications, 6, e16186 (2017).
Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy[J]. LSA, 2017,6(1). DOI： 10.1038/lsa.2016.186.

Omar, M. et al. Optical imaging of post-embryonic zebrafish using multi orientation raster scan optoacoustic mesoscopy. Light: Science & Applications, 6, e16186 (2017). DOI： 10.1038/lsa.2016.186.

摘要

Abstract

Whole-body optical imaging of post-embryonic stage model organisms is a challenging and long sought-after goal. It requires a combination of high-resolution performance and high-penetration depth. Optoacoustic (photoacoustic) mesoscopy holds great promise

as it penetrates deeper than optical and optoacoustic microscopy while providing high-spatial resolution. However

optoacoustic mesoscopic techniques only offer partial visibility of oriented structures

such as blood vessels

due to a limited angular detection aperture or the use of ultrasound frequencies that yield insufficient resolution. We introduce 360° multi orientation (multi-projection) raster scan optoacoustic mesoscopy (MORSOM) based on detecting an ultra-wide frequency bandwidth (up to 160 MHz) and weighted deconvolution to synthetically enlarge the angular aperture. We report unprecedented isotropic in-plane resolution at the 9–17 μm range and improved signal to noise ratio in phantoms and opaque 21-day-old Zebrafish. We find that MORSOM performance defines a new operational specification for optoacoustic mesoscopy of adult organisms

with possible applications in the developmental biology of adulthood and aging.

关键词

Keywords

deconvolutiondevelopmentmesoscopymultiviewoptoacousticsphotoacoustics

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