Long-term <italic style="font-style: italic">in vivo</italic> single-cell lineage tracing of deep structures using three-photon activation

Isil Tekeli; Isabelle Aujard; Xavier Trepat; Ludovic Jullien; Angel Raya; Dobryna Zalvidea

doi:10.1038/lsa.2016.84

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Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation

Original Articles

- Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation
- Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation
- LSA 2016年5卷第3期
- Affiliations：
  
  1.Center of Regenerative Medicine in Barcelona (CMRB), Barcelona Biomedical Research Park, Dr Aiguader 88, 08003 Barcelona, Spain
  2.Control of Stem Cell Potency Group, Institute for Bioengineering of Catalonia (IBEC), Barcelona Science Park, Baldiri Reixac 15-21, 08028 Barcelona, Spain
  3.École Normale Supérieure—PSL Research University, Department of Chemistry, 24 rue Lhomond, F-75005 Paris, France
  4.Sorbonne Universités, UPMC Univ Paris 06, PASTEUR, F-75005 Paris, France
  5.CNRS, UMR 8640 PASTEUR, F-75005 Paris, France
  6.Integrative Cell and Tissue Dynamics Group, Institute of Bioengineering of Catalonia (IBEC), 08028 Barcelona, Spain
  7.Institució Catalana de Recerca i Estudis Avançats (ICREA), 08010 Barcelona, Spain
  8.Center for Networked Biomedical Research on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain
- Author bio：
  
  A Raya, Email: araya@cmrb.eu
  D Zalvidea, Email: dzalvidea@ibecbarcelona.eu
- Funds：
- DOI：10.1038/lsa.2016.84
  中图分类号：
- 纸质出版日期：2016-09，
  
  网络出版日期：2016-01-29，
  
  收稿日期：2015-07-09，
  
  修回日期：2016-01-25，
  
  录用日期：2016-01-27
- Accepted：
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Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation[J]. LSA, 2016,5(3).

Tekeli, I. et al. Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation. Light: Science & Applications, 5, e16084 (2016).
Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation[J]. LSA, 2016,5(3). DOI： 10.1038/lsa.2016.84.

Tekeli, I. et al. Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation. Light: Science & Applications, 5, e16084 (2016). DOI： 10.1038/lsa.2016.84.

摘要

Abstract

Genetic labeling techniques allow for n

oninvasive lineage tracing of cells

in vivo

. Two-photon inducible activators provide spatial resolution for superficial cells

but labeling cells located deep within tissues is precluded by scattering of the far-red illumination required for two-photon photolysis. Three-photon illumination has been shown to overcome the limitations of two-photon microscopy for

in vivo

imaging of deep structures

but whether it can be used for photoactivation remains to be tested. Here we show

both theoretically and experimentally

that three-photon illumination overcomes scattering problems by combining longer wavelength excitation with high uncaging three-photon cross-section molecules. We prospectively labeled heart muscle cells in zebrafish embryos and found permanent labeling in their progeny in adult animals with negligible tissue damage. This technique allows for a noninvasive genetic manipulation

in vivo

with spatial

temporal and cell-type specificity

and may have wide applicability in experimental biology.

关键词

Keywords

multi-photon microscopyphotoactivationthree-photon microscopyzebrafish

references

Buckingham ME, Meilhac SM. Tracing cells for tracking cell lineage and clonal behavior.Dev Cell2011;21: 394–409..

Weber T, Köster R. Genetic tools for multicolor imaging in zebrafish larvae.Methods2013;62: 279–291..

Bugai LJ, Choksi AT, Mesuda CK, Kane RS, Schaffer DV. Optogenetic protein clustering and signaling activation in mammalian cells.Nat Methods2013;10: 249–252..

Madisen L, Mao T, Koch H, Zhuo JM, Berenyi Aet al. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing.Nat Neurosci2012;15: 793–802..

Gautier A, Gauron C, Volovitch M, Bensimon D, Jullien Let al. How to control proteins with light in living systems.Nat Chem Biol2014;10: 533–541..

Ellis-Davies GCR. Caged compounds: photorelease technology for control of cellular chemistry and physiology.Nat Methods2007;4: 619–628..

Feil R, Brocard J, Mascrez B, LeMeur M, Metzger Det al. Ligand-activated site-specific recombination in mice.Proc Natl Acad Sci USA1996;93: 10887–10890..

Pan XF, Wan HY, Chia W, Tong Y, Gong ZY. Demonstration of site-directed recombination in transgenic zebrafish using the Cre/loxP system.Transgenic Res2005;14: 217–223..

Sinha DK, Neveu P, Gagey N, Aujard I, Le Saux Tet al. Photoactivation of the CreERT2recombinase for conditional site-specific recombination with high spatiotemporal resolution.Zebrafish2010;7: 199–204..

Sinha DK, Neveu P, Gagey N, Aujard I, Benbrahim-Bouzidi Cet al. Photocontrol of protein activity in cultured cells and zebrafish with one- and two-photon illumination.ChemBioChem2010;11: 653–663..

Kobat D, Durst ME, Nishimura N, Wong AW, Schaffer CBet al. Deep tissue multiphoton microscopy using longer wavelength excitation.Opt Express2009;17: 13354–13364..

Horton NG, Wang K, Kobat D, Clark CG, Wise FWet al.In vivothree-photon microscopy of subcortical structures within an intact mouse brain.Nat Photonics2013;7: 205–209..

Lakowicz JR.Topics in Fluorescence Spectroscopy: Volume 5. Nonlinear and Two-photon-Induced Fluorescence. New York: Plenum Press; 1997.

Westerfield M.The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio Rerio)4th ed. Press, Eugene: Univ. of Oregon Press; 2000.

Jopling C, Sleep E, Raya M, Martí M, Raya Aet al. Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation.Nature2010;464: 606–609..

Boniface EJ, Lu JJ, Victoroff T, Zhu MY, Chen WB. FlEx-based transgenic reporter lines for visualization of Cre and Flp activity in live zebrafish.Genesis2009;47: 484–491..

Karlsson J, von Hofsten J, Olsson PE. Generating transparent zebrafish: a refined method to improve detection of gene expression during embryonic development.Mar Biotechnol2001;3: 522–527..

Neveu P, Aujard I, Benbrahim C, Le Saux T, Allemand JFet al. A caged retinoic acid for one- and two-photon excitation in zebrafish embryos.Angew Chem Int Ed2008;47: 3744–3746..

Sander V, Suñe G, Jopling C, Morera C, Izpisua Belmonte JC. Isolation andin vitroculture of primary cardiomyocytes from adult zebrafish hearts.Nat Protoc2013;8: 800–809..

Vogel A, Noack J, Hüttman G, Paltauf G. Mechanisms of femtosecond laser nanosurgery of cells and tissues.Appl Phys B2005;81: 1015–1047..

Aujard I, Benbrahim C, Gouget M, Ruel O, Baudin JBet al.o-nitrobenzyl photolabile protecting groups with red-shifted absorption: syntheses and uncaging cross-sections for one- and two-photon excitation.Chem Eur J2006;12: 6865–6879..

Bort G, Gallavardin T, Ogden D, Dalko PI. From one-photon to two-photon probes: "caged" compounds, actuators, and photoswitches.Angew Chem Int Ed2013;52: 4526–4537..

Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+indicators with greatly improved fluorescence properties.J Biol Chem1985;260: 3440–3450..

Kiskin NI, Chillingworth R, McCray JA, Piston D, Ogden D. The efficiency of two-photon photolysis of a "caged" fluorophore,o-1-(2-nitrophenyl)ethylpyranine, in relation to photodamage of synaptic terminals.Eur Biophys J2002;30: 588–604..

Gupta V, Poss KD. Clonally dominant cardiomyocytes direct heart morphogenesis.Nature2012;484: 479–484..

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Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation

Long-term in vivo single-cell lineage tracing of deep structures using three-photon activation

DOI：10.1038/lsa.2016.84

摘要

Abstract

关键词

Keywords

references