1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, China
2.MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China
D Zhu, Email: dawnzh@mail.hust.edu.cn
TH Xu, Email: xutonghui@hust.edu.cn
纸质出版日期:2018,
网络出版日期:2018-02-23,
收稿日期:2017-10-03,
修回日期:2017-10-27,
录用日期:2017-10-27
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Skull optical clearing window for
Zhao, Y. J. et al. Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution. Light: Science & Applications, 1, 17153 (2018).
Skull optical clearing window for
Zhao, Y. J. et al. Skull optical clearing window for in vivo imaging of the mouse cortex at synaptic resolution. Light: Science & Applications, 1, 17153 (2018). DOI: 10.1038/lsa.2017.153.
Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena. Here
we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution. Combined with two-photon microscopy
this technique allowed us to repeatedly image neurons
microglia and microvasculature of mice. We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation. Given its easy handling and safety
this method holds great promise for application in neuroscience research.
cranial windowdendritic spinesoptical clearingsynaptic resolutiontwo-photon imaging
Feng GP, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QTet al. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.Neuron2000;28: 41–51..
Denk W, Strickler JH, Webb WW. Two-photon laser scanning fluorescence microscopy.Science1990;248: 73–76..
Zipfel WR, Williams RM, Webb WW. Nonlinear magic: multiphoton microscopy in the biosciences.Nat Biotechnol2003;21: 1369–1377..
Svoboda K, Yasuda R. Principles of two-photon excitation microscopy and its applications to neuroscience.Neuron2006;50: 823–839..
So PT, Dong CY, Masters BR, Berland KM. Two-photon excitation fluorescence microscopy.Annu Rev Biomed Eng2000;2: 399–429..
Helmchen F, Fee MS, Tank DW, Denk W. A miniature head-mounted two-photon microscope: high-resolution brain imaging in freely moving animals.Neuron2001;31: 903–912..
Perillo EP, Jarrett JW, Liu Y-L, Hassan A, Fernée DCet al. Two-color multiphotonin vivoimaging with a femtosecond diamond raman laser.Light Sci Appl2017; e17095, doi:10.1038/lsa.2017.95..
Bar-Noam AS, Farah N, Shoham S. Correction-free remotely scanned two-photonin vivomouse retinal imaging.Light Sci Appl2016;5: e16007; doi:10.1038/lsa.2016.7..
Helmchen F, Denk W. Deep tissue two-photon microscopy.Nat Methods2005;2: 932–940..
Kneipp M, Turner J, Estrada H, Rebling J, Shoham Set al. Effects of the murine skull in optoacoustic brain microscopy.J Biophotonics2016;9: 117–123..
Fan XF, Zheng WT, Singh DJ. Light scattering and surface plasmons on small spherical particles.Light Sci Appl2014;3: e179; doi:10.1038/lsa.2014.60..
Holtmaat A, Bonhoeffer T, Chow DK, Chuckowree J, De Paola Vet al. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window.Nat Protoc2009;4: 1128–1144..
Levasseur JE, Wei EP, Raper AJ, Kontos AA, Patterson JL. Detailed description of a cranial window technique for acute and chronic experiments.Stroke1975;6: 308–317..
Yang G, Pan F, Parkhurst CN, Grutzendler J, Gan WB. Thinned-skull cranial window technique for long-term imaging of the cortex in live mice.Nat Protoc2010;5: 201–208..
Yu XZ, Zuo Y. Two-photonin vivoimaging of dendritic spines in the mouse cortex using a thinned-skull preparation.J Vis Exp2014;87: e51520..
Takehara H, Nagaoka A, Noguchi J, Akagi T, Kasai Het al. Lab-on-a-brain: implantable micro-optical fluidic devices for neural cell analysisin vivo.Sci Rep2014;4: 6721..
Roome CJ, Kuhn B. Chronic cranial window with access port for repeated cellular manipulations, drug application, and electrophysiology.Front Cell Neurosci2014;8: 379..
Goldey GJ, Roumis DK, Glickfeld LL, Kerlin AM, Reid RCet al. Removable cranial windows for long-term imaging in awake mice.Nat Protoc2014;9: 2515–2538..
Drew PJ, Shih AY, Driscoll JD, Knutsen PM, Blinder Pet al. Chronic optical access through a polished and reinforced thinned skull.Nat Methods2010;7:981–984..
Dombeck D, Tank D. Two-photon imaging of neural activity in awake mobile mice.Cold Spring Harb Protoc2014;2014: 726–736..
Nimmerjahn A. Optical window preparation for two-photon imaging of microglia in mice.Cold Spring Harb Protoc2012;2012: 587–593..
Shih AY, Drew PJ, Kleinfeld D. Imaging vasodynamics in the awake mouse brain with two-photon microscopy. In: Zhao M, Ma H, Schwartz TH, editors.Neurovascular Coupling Methods. Neuromethods. New York: Humana Press; 2014. pp55–73.
Heo C, Park H, Kim YT, Baeg E, Kim YHet al. A soft, transparent, freely accessible cranial window for chronic imaging and electrophysiology.Sci Rep2016;6: 27818..
Dorand RD, Barkauskas DS, Evans TA, Petrosiute A, Huang AY. Comparison of intravital thinned skull and cranial window approaches to study CNS immunobiology in the mouse cortex.Intravital2014;3: e29728..
Portera-Cailliau C, Weimer RM, De Paola V, Caroni P, Svoboda K. Diverse modes of axon elaboration in the developing neocortex.PLoS Biol2005;3: e272..
Cruz-Martin A, Portera-Cailliau C.In vivoimaging of axonal and dendritic structures in neonatal mouse cortex.Cold Spring Harb Protoc2014;2014: 57–64..
Kim TH, Zhang YP, Lecoq J, Jung JC, Li JEet al. Long-term optical access to an estimated one million neurons in the live mouse cortex.Cell Rep2016;17: 3385–3394..
Shih AY, Mateo C, Drew PJ, Tsai PS, Kleinfeld D. A polished and reinforced thinned-skull window for long-term imaging of the mouse brain.J Vis Exp2012;61: e3742..
Xu HT, Pan F, Yang G, Gan WB. Choice of cranial window type forin vivoimaging affects dendritic spine turnover in the cortex.Nat Neurosci2007;10: 549–551..
Zhu D, Larin KV, Luo QM, Tuchin VV. Recent progress in tissue optical clearing.Laser Photonics Rev2013;7: 732–757..
Wang J, Zhang Y, Li PC, Luo QM, Zhu D. Review: tissue optical clearing window for blood flow monitoring.IEEE J Sel Top Quant Electron2014;20: 6801112..
Chung K, Deisseroth K. CLARITY for mapping the nervous system.Nat Methods2013;10: 508–513..
Tainaka K, Kubota SI, Suyama TQ, Susaki EA, Perrin Det al. Whole-body imaging with single-cell resolution by tissue decolorization.Cell2014;159: 911–924..
Yang B, Treweek JB, Kulkarni RP, Deverman BE, Chen CKet al. Single-cell phenotyping within transparent intact tissue through whole-body clearing.Cell2014;158: 945–958..
Ertürk A, Becker K, Jährling N, Mauch CP, Hojer CDet al. Three-dimensional imaging of solvent-cleared organs using 3DISCO.Nat Protoc2012;7: 1983–1995..
Pan CC, Cai RY, Quacquarelli FP, Ghasemigharagoz A, Lourbopoulos Aet al. Shrinkage-mediated imaging of entire organs and organisms using uDISCO.Nat Methods2016;13: 859–867..
Lee E, Choi J, Jo Y, Kim JY, Jang YJet al. ACT-PRESTO: rapid and consistent tissue clearing and labeling method for 3-dimensional (3D) imaging.Sci Rep2016;6: 18631..
Renier N, Adams EL, Kirst C, Wu ZH, Azevedo Ret al. Mapping of brain activity by automated volume analysis of immediate early genes.Cell2016;165: 1789–1802..
Berke IM, Miola JP, David MA, Smith MK, Price C. Seeing through musculoskeletal tissues: improvingin situimaging of bone and the lacunar canalicular system through optical clearing.PLoS One2016;11: e0150268..
Neu CP, Novak T, Gilliland KF, Marshall P, Calve S. Optical clearing in collagen- and proteoglycan-rich osteochondral tissues.Osteoarthr Cartil2015;23: 405–413..
Greenbaum A, Chan KY, Dobreva T, Brown D, Balani DHet al. Bone CLARITY: clearing, imaging, and computational analysis of osteoprogenitors within intact bone marrow.Sci Transl Med2017;9: eaah6518..
Wang J, Zhang Y, Xu TH, Luo QM, Zhu D. An innovative transparent cranial window based on skull optical clearing.Laser Phys Lett2012;9: 469–473..
Yang XQ, Zhang Y, Zhao K, Zhao YJ, Liu YYet al. Skull optical clearing solution for enhancing ultrasonic andphotoacoustic imaging.IEEE Trans Med Imag2016;35: 1903–1906..
Kitaura H, Hishida R, Shibuki K. Transcranial imaging of somatotopic map plasticity after tail cut in mice.Brain Res2010;1319: 54–59..
Pekny M, Nilsson M. Astrocyte activation and reactive gliosis.Glia2005;50: 427–434..
Zuo Y, Lin A, Chang P, Gan WB. Development of long-term dendritic spine stability in diverse regions of cerebral cortex.Neuron2005;46: 181–189..
Holtmaat AJGD, Trachtenberg JT, Wilbrecht L, Shepherd GM, Zhang XQet al. Transient and persistent dendritic spines in the neocortexin vivo.Neuron2005;45: 279–291..
Davalos D, Grutzendler J, Yang G, Kim JV, Zuo Yet al. ATP mediates rapid microglial response to local brain injuryin vivo.Nat Neurosci2005;8: 752–758..
Nimmerjahn A, Kirchhoff F, Helmchen F. Resting microglial cells are highly dynamic surveillants of brain parenchymain vivo.Science2005;308: 1314–1318..
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