Super-resolution visible photoactivated atomic force microscopy

Seunghyun Lee; Owoong Kwon; Mansik Jeon; Jaejung Song; Seungjun Shin; HyeMi Kim; Minguk Jo; Taiuk Rim; Junsang Doh; Sungjee Kim; Junwoo Son; Yunseok Kim; Chulhong Kim

doi:10.1038/lsa.2017.80

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Super-resolution visible photoactivated atomic force microscopy

Original Articles

- Super-resolution visible photoactivated atomic force microscopy
- Super-resolution visible photoactivated atomic force microscopy
- LSA 2017年6卷第4期
- Affiliations：
  
  1.Future IT Innovation Laboratory, Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
  2.School of Advanced Materials Science and Engineering, Sungkyunkwan University (SKKU), Suwon, Republic of Korea
  3.School of Electronics Engineering, Kyungpook National University (KNU), Daegu, Republic of Korea
  4.School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
  5.Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
  6.Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
  7.Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
  8.Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
- Author bio：
  
  Y Kim, Email: yunseokkim@skku.edu
  C Kim, Email: chulhong@postech.edu
- Funds：
- DOI：10.1038/lsa.2017.80
  中图分类号：
- 纸质出版日期：2017-12，
  
  网络出版日期：2017-05-04，
  
  收稿日期：2016-11-23，
  
  修回日期：2017-04-26，
  
  录用日期：2017-05-01
- Accepted：
Scan QR Code
Super-resolution visible photoactivated atomic force microscopy[J]. LSA, 2017,6(4).

Lee, S. et al. Super-resolution visible photoactivated atomic force microscopy. Light: Science & Applications, 6, e17080 (2017).
Super-resolution visible photoactivated atomic force microscopy[J]. LSA, 2017,6(4). DOI： 10.1038/lsa.2017.80.

Lee, S. et al. Super-resolution visible photoactivated atomic force microscopy. Light: Science & Applications, 6, e17080 (2017). DOI： 10.1038/lsa.2017.80.

摘要

Abstract

Imaging the intrinsic optical absorption properties of nanomaterials with optical microscopy (OM) is hindered by the optical diffraction limit and intrinsically poor sensitivity. Thus

expensive and destructive electron microscopy (EM) has been commonly used to examine the morphologies of nanostructures. Further

while nanoscale fluorescence OM has become crucial for investigating the morphologies and functions of intracellular specimens

this modality is not suitable for imaging optical absorption and requires the use of possibly undesirable exogenous fluorescent molecules for biological samples. Here we demonstrate super-resolution visible photoactivated atomic force microscopy (pAFM)

which can sense intrinsic optical absorption with ~8 nm resolution. Thus

the resolution can be improved down to ~8 nm. This system can detect not only the first harmonic response

but also the higher harmonic response using the nonlinear effect. The thermoelastic effects induced by pulsed laser irradiation allow us to obtain visible pAFM images of single gold nanospheres

various nanowires

and biological cells

all with nanoscale resolution. Unlike expensive EM

the visible pAFM system can be simply implemented by adding an optical excitation sub-system to a commercial atomic force microscope.

关键词

Keywords

Arabidopsis imaginggold nanoparticle imagingmelanoma cell imagingnanowire imagingsuper-resolution optical microscopy

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