Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration

Dong-Yu Li; Qing Xia; Ting-Ting Yu; Jing-Tan Zhu; Dan Zhu

doi:10.1038/s41377-021-00682-8

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Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration

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- Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration
- Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration
- LSA 2021年10卷第12期页码：2492-2506
- Affiliations：
  
  1.Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China
  2.MoE Key Laboratory for Biomedical Photonics, Huazhong University of Science and Technology, 430074 Wuhan, Hubei, China
- Author bio：
  
  Dan Zhu (dawnzh@mail.hust.edu.cn)
- Funds：
- DOI：10.1038/s41377-021-00682-8
  中图分类号：
- 纸质出版日期：2021-12-31，
  
  网络出版日期：2021-12-03，
  
  收稿日期：2021-06-25，
  
  修回日期：2021-10-20，
  
  录用日期：2021-11-23
- Accepted：
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Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration[J]. LSA, 2021,10(12):2492-2506.

Li, D. Y. et al. Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration. Light: Science & Applications, 10, 2492-2506 (2021).
Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration[J]. LSA, 2021,10(12):2492-2506. DOI： 10.1038/s41377-021-00682-8.

Li, D. Y. et al. Transmissive-detected laser speckle contrast imaging for blood flow monitoring in thick tissue: from Monte Carlo simulation to experimental demonstration. Light: Science & Applications, 10, 2492-2506 (2021). DOI： 10.1038/s41377-021-00682-8.

摘要

Abstract

Laser speckle contrast imaging (LSCI) is a powerful tool to monitor blood flow distribution and has been widely used in studies of microcirculation

both for animal and clinical applications. Conventionally

LSCI usually works on reflective-detected mode. However

it could provide promising temporal and spatial resolution for in vivo applications only with the assistance of various tissue windows

otherwise

the overlarge superficial static speckle would extremely limit its contrast and resolution. Here

we systematically investigated the capability of transmissive-detected LSCI (TR-LSCI) for blood flow monitoring in thick tissue. Using Monte Carlo simulation

we theoretically compared the performance of transmissive and reflective detection. It was found that the reflective-detected mode was better when the target layer was at the very surface

but the imaging quality would rapidly decrease with imaging depth

while the transmissive-detected mode could obtain a much stronger signal-to-background ratio (SBR) for thick tissue. We further proved by tissue phantom

animal

and human experiments that in a certain thickness of tissue

TR-LSCI showed remarkably better performance for thick-tissue imaging

and the imaging quality would be further improved if the use of longer wavelengths of near-infrared light. Therefore

both theoretical and experimental results demonstrate that TR-LSCI is capable of obtaining thick-tissue blood flow information and holds great potential in the field of microcirculation research.

关键词

Keywords

references

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