Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy

Fei Yang; Zhiyang Wang; Wenbin Shi; Miao Wang; Rui Ma; Wuyu Zhang; Xipeng Li; Erqi Wang; Wenjie Xie; Zhan Zhang; Qi Shen; Feifan Zhou; Sihua Yang

doi:10.1038/s41377-024-01450-0

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Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy

Original Articles

- Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy
- Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy
- Light: Science & Applications 2024年13卷第5期页码：894-907
- Affiliations：
  
  1.MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, China
  2.Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
  3.Key Laboratory of Biomedical Engineering of Hainan Province, School of Biomedical Engineering, Hainan University, Haikou 570100, China
  4.Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  5.Guangdong Province Key Laboratory of Brain Function and Disease, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510120, China
  6.Guangdong Basic Research Center of Excellence for Structure and Fundamental Interactions of Matter, South China Normal University, Guangzhou 510006, China
- Author bio：
  
  Qi Shen (shenqi@scnu.edu.cn)
  Feifan Zhou (zhouff@hainanu.edu.cn)
  Sihua Yang (yangsh@scnu.edu.cn)
- Funds：
- DOI：10.1038/s41377-024-01450-0
  中图分类号：
- 收稿：2023-10-09，
  
  修回：2024-03-24，
  
  录用：2024-04-09，
  
  网络出版：2024-04-25，
  
  纸质出版：2024-05-31
- Accepted：
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Fei Yang, Zhiyang Wang, Wenbin Shi, 等. Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy[J]. Light: Science & Applications, 2024,13(5):894-907.

Yang, F. et al. Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy. Light: Science & Applications, 13, 894-907 (2024).
Fei Yang, Zhiyang Wang, Wenbin Shi, 等. Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy[J]. Light: Science & Applications, 2024,13(5):894-907. DOI： 10.1038/s41377-024-01450-0.

Yang, F. et al. Advancing insights into in vivo meningeal lymphatic vessels with stereoscopic wide-field photoacoustic microscopy. Light: Science & Applications, 13, 894-907 (2024). DOI： 10.1038/s41377-024-01450-0.

摘要

Abstract

Meningeal lymphatic vessels (mLVs) play a pivotal role in regulating metabolic waste from cerebrospinal fluid (CSF). However

the current limitations in field of view and resolution of existing imaging techniques impede understanding the stereoscopic morphology and dynamic behavior of mLVs in vivo. Here

we utilized dual-contrast functional photoacoustic microscopy to achieve wide-field intravital imaging of the lymphatic system

including mLVs and glymphatic pathways. The stereoscopic photoacoustic microscopy based on opto-acoustic confocal features has a depth imaging capability of 3.75 mm

facilitating differentiation between mLVs on the meninges and glymphatic pathways within the brain parenchyma. Subsequently

using this imaging technique

we were able to visualize the dynamic drainage of mLVs and identify a peak drainage period occurring around 20–40 min after injection

along with determining the flow direction from CSF to lymph nodes. Inspiringly

in the Alzheimer's disease (AD) mouse model

we observed that AD mice exhibit a ~ 70% reduction in drainage volume of mLVs compared to wild-type mice. With the development of AD

there is be continued decline in mLVs drainage volume. This finding clearly demonstrates that the AD mouse model has impaired CSF drainage. Our study opens up a horizon for understanding the brain's drainage mechanism and dissecting mLVs-associated neurological disorders.

关键词

Keywords

references

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