Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)

Xinzhu Xu; Wenyi Wang; Liang Qiao; Yunzhe Fu; Xichuan Ge; Kun Zhao; Karl Zhanghao; Meiling Guan; Xin Chen; Meiqi Li; Dayong Jin; Peng Xi

doi:10.1038/s41377-024-01464-8

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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)

Original Articles

- Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)
- Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)
- LSA 2024年13卷第6期页码：1140-1158
- Affiliations：
  
  1.Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing 100871, China
  2.Wallace H. Coulter Dept. of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta 30332 GA, USA
  3.National Biomedical Imaging Center, Peking University, Beijing 100871, China
  4.Department of Biomedical Engineering, College of Engineering, Southern University of Science and Technology, Shenzhen 518055, Guangdong, China
  5.Airy Technologies Co., Ltd., Beijing 100086, China
  6.Eastern Institute for Advanced Study, Eastern Institute of Technology, Ningbo, Zhejiang 315200, China
  7.School of Life Science, Peking University, Beijing 100871, China
  8.Institute for Biomedical Materials and Devices (IBMD), Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
- Author bio：
  
  Dayong Jin (Dayong.Jin@uts.edu.au)
  Peng Xi (xipeng@pku.edu.cn)
- Funds：
- DOI：10.1038/s41377-024-01464-8
  中图分类号：
- 收稿：2023-10-10，
  
  修回：2024-04-08，
  
  录用：2024-04-24，
  
  网络出版：2024-05-29，
  
  纸质出版：2024-06-30
- Accepted：
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Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)[J]. LSA, 2024,13(6):1140-1158.

Xu, X. Z. et al. Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)., Light: Science & Applications, 13, 1140-1158 (2024).
Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)[J]. LSA, 2024,13(6):1140-1158. DOI： 10.1038/s41377-024-01464-8.

Xu, X. Z. et al. Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM)., Light: Science & Applications, 13, 1140-1158 (2024). DOI： 10.1038/s41377-024-01464-8.

摘要

Abstract

Structured illumination microscopy (SIM) has emerged as a promising super-resolution fluorescence imaging technique

offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological specimens. Traditional efforts to enhance system frame rates have concentrated on processing algorithms

like rolling reconstruction or reduced frame reconstruction

or on investments in costly sCMOS cameras with accelerated row readout rates. In this article

we introduce an approach to elevate SIM frame rates and region of interest (ROI) coverage at the hardware level

without necessitating an upsurge in camera expenses or intricate algorithms. Here

parallel acquisition-readout SIM (PAR-SIM) achieves the highest imaging speed for fluorescence imaging at currently available detector sensitivity. By using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process

we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels · s

−1

9.6-fold that of the latest techniques

with the lowest SNR of −2.11 dB and 100 nm resolution. PAR-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations

even under conditions of low signal due to ultra-short exposure times. Notably

mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell

recorded with PAR-SIM at an impressive 408 Hz. We posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词

Keywords

references

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