Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart

Guoshuai Zhu; Jianyun Xiong; Xing Li; Ziyi He; Shuhan Zhong; Junlin Chen; Yang Shi; Ting Pan; Li Zhang; Baojun Li; Hongbao Xin

doi:10.1038/s41377-024-01617-9

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Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart

Original Articles

- Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart
- Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart
- LSA 2024年13卷第11期页码：2668-2679
- Affiliations：
  
  1.Guangdong Provincial Key Laboratory of Nanophotonic Manipulation, Institute of Nanophotonics, College of Physics and Optoelectronic Engineering, Jinan University, Guangzhou 511443, China
  2.Key Laboratory of CNS Regeneration (Ministry of Education), Guangdong-Hong Kong-Macau Institute of CNS Regeneration, Jinan University, Guangzhou 510632, China
- Author bio：
  
  Baojun Li (baojunli@jnu.edu.cn)
  Hongbao Xin (hongbaoxin@jnu.edu.cn)
- Funds：
- DOI：10.1038/s41377-024-01617-9
  中图分类号：
- 收稿日期：2024-03-29，
  
  修回日期：2024-08-26，
  
  录用日期：2024-08-29，
  
  网络出版日期：2024-09-19，
  
  纸质出版日期：2024-11-30
- Accepted：
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Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart[J]. LSA, 2024,13(11):2668-2679.

Zhu, G. S. et al. Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart. Light: Science & Applications, 13, 2668-2679 (2024).
Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart[J]. LSA, 2024,13(11):2668-2679. DOI： 10.1038/s41377-024-01617-9.

Zhu, G. S. et al. Neural stimulation and modulation with sub-cellular precision by optomechanical bio-dart. Light: Science & Applications, 13, 2668-2679 (2024). DOI： 10.1038/s41377-024-01617-9.

摘要

Abstract

Neural stimulation and modulation at high spatial resolution are crucial for mediating neuronal signaling and plasticity

aiding in a better understanding of neuronal dysfunction and neurodegenerative diseases. However

developing a biocompatible and precisely controllable technique for accurate and effective stimulation and modulation of neurons at the subcellular level is highly challenging. Here

we report an optomechanical method for neural stimulation and modulation with subcellular precision using optically controlled bio-darts. The bio-dart is obtained from the tip of sunflower pollen grain and can generate transient pressure on the cell membrane with submicrometer spatial resolution when propelled by optical scattering force controlled with an optical fiber probe

which results in precision neural stimulation via precisely activation of membrane mechanosensitive ion channel. Importantly

controllable modulation of a single neuronal cell

even down to subcellular neuronal structures such as dendrites

axons

and soma

can be achieved. This bio-dart can also serve as a drug delivery tool for multifunctional neural stimulation and modulation. Remarkably

our optomechanical bio-darts can also be used for in vivo neural stimulation in larval zebrafish. This strategy provides a novel approach for neural stimulation and modulation with sub-cellular precision

paving the way for high-precision neuronal plasticity and neuromodulation.

关键词

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