In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications

Hyejin Chang; Won Hur; Homan Kang; Bong-Hyun Jun

doi:10.1038/s41377-024-01718-5

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In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications

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- In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications
- In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications
- LSA 2025年14卷第3期页码：610-633
- Affiliations：
  
  1.Division of Science Education, Kangwon National University, Chuncheon 24341, South Korea
  2.Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
  3.Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, South Korea
- Author bio：
  
  Homan Kang (hkang7@mgh.harvard.edu)
  Bong-Hyun Jun (bjun@konkuk.ac.kr)
- Funds：
- DOI：10.1038/s41377-024-01718-5
  中图分类号：
- 收稿日期：2024-07-23，
  
  修回日期：2024-11-29，
  
  录用日期：2024-12-15，
  
  网络出版日期：2025-02-11，
  
  纸质出版日期：2025-03-31
- Accepted：
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In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications[J]. LSA, 2025,14(3):610-633.

Chang, H. J. et al. In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications. Light: Science & Applications, 14, 610-633 (2025).
In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications[J]. LSA, 2025,14(3):610-633. DOI： 10.1038/s41377-024-01718-5.

Chang, H. J. et al. In vivo surface-enhanced Raman scattering techniques: nanoprobes, instrumentation, and applications. Light: Science & Applications, 14, 610-633 (2025). DOI： 10.1038/s41377-024-01718-5.

摘要

Abstract

Surface-enhanced Raman scattering (SERS) has emerged as a powerful tool in various biomedical applications

including in vivo imaging

diagnostics

and therapy

largely due to the development of near-infrared (NIR) active SERS substrates. This review provides a comprehensive overview of SERS-based applications in vivo

focusing on key aspects such as the design considerations for SERS nanoprobes and advancements in instrumentation. Topics covered include the development of NIR SERS substrates

Raman label compounds (RLCs)

protective coatings

and the conjugation of bioligands for targeted imaging and therapy. The review also discusses microscope-based configurations such as scanning

widefield imaging

and fiber-optic setups. Recent advances in using SERS nanoprobes for in vivo sensing

diagnostics

biomolecule screening

multiplex imaging

intraoperative guidance

and multifunctional cancer therapy are highlighted. The review concludes by addressing challenges in the clinical translation of SERS nanoprobes and outlines future directions

emphasizing opportunities for advancing biomedical research and clinical applications.

关键词

Keywords

references

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